| 百里香愈伤组织的诱导及细胞悬浮培养体系的建立 |
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| 引用本文： | 徐世千,李晓东,张建国. 百里香愈伤组织的诱导及细胞悬浮培养体系的建立[J]. 西北农业学报, 2011, 20(10): 112-119 |
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| 作者姓名： | 徐世千 李晓东 张建国 |
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| 作者单位： | 华南农业大学农学院;深圳职业技术学院化生学院; |
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| 基金项目： | 深圳市重点科技项目(2109k3070009) |
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| 摘 要： | 以普通百里香无菌苗的茎段和叶片为材料,对百里香愈伤组织诱导和继代增殖的最佳培养条件进行研究,并对悬浮细胞的生长特性进行监测,初步建立细胞悬浮培养体系.结果表明,茎段是诱导愈伤组织和进行悬浮培养的理想外植体,愈伤组织最佳诱导培养基为MS+NAA 1.0 mg/L+6-BA 0.5 mg/L+2,4-D0.5 mg/L,最...
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| 关 键 词： | 百里香 愈伤组织 悬浮培养 生长特性 |
Callus Induction and Establishment of Cell Suspension Culture System for Thymus vulgaris L. |
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| XU Shiqian,LI Xiaodong and ZHANG Jianguo. Callus Induction and Establishment of Cell Suspension Culture System for Thymus vulgaris L.[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2011, 20(10): 112-119 |
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| Authors: | XU Shiqian LI Xiaodong ZHANG Jianguo |
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| Affiliation: | XU Shiqian1,2,LI Xiaodong2 and ZHANG Jianguo1(1.College of Agriculture,South China Agriculture University,Guangzhou 510642,China,2.School of Applied Chemical and Biological Technology,Shenzhen Polytechnic,Shenzhen 518055,China) |
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| Abstract: | The stems and leaves of aseptic seedling of Thymus vulgaris L.were taken as explants to study the optimum culture conditions of callus induction and proliferation.In addition,the growth characteristics of suspension cell were investigated and the system of thymus cell suspension culture was established preliminarily.The results showed that stem explants were the most suitable for callus induction,the optimal medium for callus induction was MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L+ 2,4-D 0.5 mg/L,the optimal formul... |
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| Keywords: | Thymus Callus Suspension culture Growth characteristics |
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