| 茶皂素的提取纯化及成品中茶皂素含量的测定 |
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| 引用本文： | 谢秋英,黄玉英,宋振荣. 茶皂素的提取纯化及成品中茶皂素含量的测定[J]. 福建水产, 2010, 0(2): 14-17 |
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| 作者姓名： | 谢秋英 黄玉英 宋振荣 |
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| 作者单位： | 集美大学水产学院,福建省高校水产疾病防治技术研发重点实验室,福建,厦门,361021 |
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| 基金项目： | 福建省海洋与渔业厅资助项目 |
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| 摘 要： | 采用AB-8大孔吸附树脂对茶皂素成品进行纯化,提取标准品。利用紫外-可见分光光度计进行扫描得出茶皂素标准品在205nm处出现最大吸收峰;经香草醛-硫酸显色,最大吸收波长为435nm,在539nm处有较弱的吸收峰。用自制茶皂素纯品为标样,采用香草醛-浓硫酸显色法,绘制茶皂素定量的标准曲线,测定成品茶皂素的含量为80.9%。
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| 关 键 词： | 茶皂素 提取 成品 |
Purification and quantitative determination of tea saponin extracted from finished product |
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| XIE Qiu-ying,HUANG Yu-ying,SONG Zhen-rong. Purification and quantitative determination of tea saponin extracted from finished product[J]. Journal of Fujian Fisheries, 2010, 0(2): 14-17 |
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| Authors: | XIE Qiu-ying HUANG Yu-ying SONG Zhen-rong |
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| Affiliation: | XIE Qiu-ying,HUANG Yu-ying,SONG Zhen-rong(Key Laboratory of Technology of Disease Prevention for Aquaculture,Fisheries College of Jimei University,Xiamen 361021,China) |
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| Abstract: | After purifying the finished tea saponin with macroporous adsorption resin AB-8,we extracted the standard samples.Scanned under the UV-visible spectrophotometer.the standard tea saponin had absorption peaks in 205nm.The colour reaction of vanillin sulfuric acid showed that the maximum absorption wavelength was 435nm,and there was a weak absorption peak in 539nm.Using vanillin sulfuric acid process,the standard curves of quantitative determination were studied with self-made tea saponin as standard samples a... |
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| Keywords: | Tea saponin extraction finished product |
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