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| 不同的血清学方法对布病疫苗免疫绵羊抗体检测结果的比较研究 | |
| 引用本文： | 蔡一非,赵智香,钟旗,何倩倪,于伟玲,黄保续,范伟兴.不同的血清学方法对布病疫苗免疫绵羊抗体检测结果的比较研究[J].中国动物检疫,2008,25(12):31-33. |
| 作者姓名： | 蔡一非 赵智香 钟旗 何倩倪 于伟玲 黄保续 范伟兴 |
| 作者单位： | 1. 新疆农业大学动物医学院,新疆乌鲁木齐,830052;中国动物卫生与流行病学中心,山东青岛,266032 2. 中国动物卫生与流行病学中心,山东青岛,266032 3. 新疆畜牧科学院兽医研究所,新疆乌鲁木齐,830000 4. 加拿大食品检疫署OIE布病参考实验室,安大略,K2H8P9 |
| 摘 要： | 将国内广泛用于预防羊布病的S2和M5疫苗免疫绵羊,然后定期采血进行细菌分离和血清学检测,并比较血清学方法的检测结果。结果表明ELISA操作方便,具有较好的敏感性和特异性,建议在检疫中用敏感性较高的RBT初筛,用特异性较好的ELISA确诊,以得到较好的效果。 |
| 关 键 词： | S2 M5 血清学检测 |
Comparision of different serological tests for detection of antibodies in sheep vaccinated with brucellosis vaccines |
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| K.Nielsen,CAI Yifei,ZHAO Zhixiang,ZHONG Qi,HE Qianni,K.Nielsen,YU Weiling,HUANG Baoxu,FAN Weixing.Comparision of different serological tests for detection of antibodies in sheep vaccinated with brucellosis vaccines[J].China Journal Of Animal Quarantine,2008,25(12):31-33. | |
| Authors: | KNielsen CAI Yifei ZHAO Zhixiang ZHONG Qi HE Qianni KNielsen YU Weiling HUANG Baoxu FAN Weixing |
| Institution: | 1. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi Xinjiang 830052; 2. China Animal Health and Epidemiology Center, Qingdao Shangdong 266032; 3. Institute of Veterinary Research, Xinjiang Academy of Animal Sciences, Urumqi Xijiang 830000; 4. Canadian Food Inspection Agency, Ontario Canada K2H8P9) |
| Abstract: | S2 and M5 vaccines broadly used in preventing caprine and ovine brucellosis were vaccinated to the sheep. Blood samples were collected at regular intervals for direct brucella isolation and serological tests , and different seralogical tests were compared. Results showed ELISA was simple in operation with better sensitivity and specificity. It was recommended that the RBT with its higher sensibility might be first used in screening test and the ELISA with its higher specificity, used to recheck in order to get more satisfactory results. |
| Keywords: | S2 M5 Serological tests |
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