| 岷江百合根尖染色体的C-分带和FISH分析 |
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| 引用本文： | 刘光欣,胡凤荣,席梦利,吴祝华,池坚,施季森.岷江百合根尖染色体的C-分带和FISH分析[J].分子植物育种,2008,6(1):95-99. |
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| 作者姓名： | 刘光欣 胡凤荣 席梦利 吴祝华 池坚 施季森 |
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| 作者单位： | 国家教育部和江苏省共建林木遗传和生物技术重点实验室,南京林业大学,南京,210037 |
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| 基金项目： | 上海市科技攻关项目
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江苏省自然科学基金
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引进国际先进农业科技计划(948计划) |
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| 摘 要： | 利用Giemsa C-分带和荧光原位杂交(FISH)的方法对岷江百合(L.regale)根尖染色体进行了研究.结果表明岷江百合的带型公式为:2n=24=4I 8CL 2I 6I 2I T 2I T ,每条染色体都显示出了可以明显区别的特征带,带纹强弱差异明显.以45S rDNA为探针对岷江百合根尖染色体进行了荧光原位杂交,杂交点数为5对,分别位于A、B、H和K 4对同源染色体的着丝点区域和一对G染色体的长臂上.通过Giemsa C-带和HSH的方法可以将岷江百合的每条染色体区分开来.
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| 关 键 词： | 岷江百合 染色体 C-带分析 荧光原位杂交 岷江百合 根尖 同源染色体 FISH 分析 Tips Root Means Chromosome Lily Analysis 区域 着丝点 荧光原位杂交 探针 rDNA 差异明显 带纹 特征 显示 |
Giemsa C-banding and FISH Analysis of Minjiang Lily Chromosome by Means of Root Tips |
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| Liu Guangxin,Hu Fengrong,Xi Mengli,Wu Zhuhua,Chi Jian,Shi Jisen.Giemsa C-banding and FISH Analysis of Minjiang Lily Chromosome by Means of Root Tips[J].Molecular Plant Breeding,2008,6(1):95-99. |
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| Authors: | Liu Guangxin Hu Fengrong Xi Mengli Wu Zhuhua Chi Jian Shi Jisen |
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| Institution: | Liu Guangxin Hu Fengrong Xi Mengli Wu Zhuhua Chi Jian Shi Jisen The Key Laboratory of Forest Genetics & Biotechnology,Ministry of Education , Jiangsu Government,Nanjing Forestry University,Nanjing,210037 |
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| Abstract: | Giemsa C-banding and FISH analysis of the Minjiang lily chromosomes (Lilium regale) from root tips were carried out in this research. The results showed that the C-banding karyotype would be 2n=24=4I+8CI++2I++6I++2I+T++2I+T+. The specific C-banding points on each chromosome could be distinguished and the differences of C-banding intensity also could be recognized. 45S rDNA was used as probe for FISH analysis (fluorescence in situ hybridization), and 5 couples of hybridization signals were displayed on the c... |
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