大蒜蒜瓣离体繁殖研究

以山东苍山大蒜蒜瓣为外植体进行离体培养,在MS+KT_1.0+NAA_0.005(单位:mg/l,下同)培养基上,外植体可直接分化出新梢.新梢在MS+KT_1.0+IBA_1.0+NAA_2.0培养基上进行2次继代增殖,然后在B₅+BA_1.0培养基上进行1次继代增殖,繁殖系数依次为7.6,6.5,4.0.共经160～180天,单瓣蒜累积繁殖系数可达1640.新梢在MS+NAA_0.1+BA_0.1培养基上生根率可达87.5%.在MS+NAA_2.0+BA_0.05培养基上,小鳞茎发生率可达85.7%.采用有根单鳞茎试管苗移栽,成活率为92%.细胞学观察表明,该途径保持了原材料的遗传稳定性.

IN VITRO PROPAGATION OF GARLIC CLOVES

In vitro propagation of garlic cloves was discussed in this paper.Proliferated shoots were produced directly when the garlic cloves pieces of Cangshan were cultured in vitro on the medium MS suplemented with 1.0 mg/L KT and 0.005 mg/L NAA.When the shoots were subcultured twice on the medium MS suplemented with 1.0 mg/L KT,1.0 mg/L IBA and 2.0 mg/L NAA,shoots could be proliferated more,Propagation coeffcient was 7.6 and 6.5.Then subcultured on the medium BS suplemented with BA 1.0 mg/L,propagation coefficient was 4.0.Through the initial culture and three times subculture during 160~180 days,total shoots propagated from one clove were 1 640.When the shoots were cultured on the medium MS suplemented with 0.1 mg/L BA and 0.1 mg/L NAA,rootability got to 87.5%.Bulblets could be formated on the medium MS suplemented with 2.0 mg/L NAA and 0.05 mg/L BA.When plantlets with roots and bulblets were transplanted,the survival rate got to 92%.Cytogenetic analysis on the tube plantlets from different generation showed that there were 16 chromosomes in all single cells observed,no variation was found.So the genetic type of primary material was maintained well.