In vitro effects of insulin on glucose and lipid metabolism in rat embryos |
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Authors: | Hirotada TSUJII Yumi NAKAMURA and Koh-ichi HAMANO |
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Institution: | Faculty of Agriculture, Shinshu University, Minamiminowa-mura, Nagano-ken, Japan |
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Abstract: | Glucose is utilized for oxidation and synthesis of various lipids in cultured rat embryos. The present experiment examined the effect of insulin on the incorporation of glucose into lipid fractions in rat embryos in vitro . Embryos at the 2-cell, 8-cell and blastocyst stages were incubated for 5 h in hamster embryo culture medium (HECM)-1 containing 14C-glucose and 170 nmol/L insulin, or in HECM-1 containing only 14C-glucose, and the oxidation of glucose in these embryos was examined. In addition, the total lipids of blastocysts were separated by thin layer chromatography and the radioactivity of the separated lipid fractions was measured. Oxidation of glucose was significantly increased after insulin treatment compared with that without insulin treatment in 8-cell embryos and blastocysts ( P < 0.05), but not in 2-cell embryos. Incorporation of glucose into lipids in blastocysts was significantly lowered by insulin treatment compared with that without insulin treatment ( P < 0.05). Most of the radioactivity was recovered from triacylglycerols of blastocysts and the remaining radioactivity was found in other neutral lipids and phospholipids. We conclude that insulin accelerates the utilization for oxidation of glucose and inhibits the storage of triacylglycerols in rat blastocysts. |
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Keywords: | glucose incorporation glucose oxidation insulin lipid synthesis rat embryo |
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