| 甘薯淀粉废水培养类球红细菌条件优化 |
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| 引用本文： | 韩庆莉,赵志瑞,周永双,王栋,白志辉.甘薯淀粉废水培养类球红细菌条件优化[J].安徽农学通报,2012,18(13):35-38. |
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| 作者姓名： | 韩庆莉 赵志瑞 周永双 王栋 白志辉 |
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| 作者单位： | 西南林业大学,云南昆明650224 中国科学院生态环境研究中心,北京100085;中国科学院生态环境研究中心,北京,100085;无锡中科活力生物技术有限公司,江苏宜兴,214200 |
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| 基金项目： | 西南林业大学博士启动基金;中国科学院知识创新工程重点部署项目 |
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| 摘 要： | 类球红细菌(Rhodobacter sphaeroides)作为微生态制剂的生产菌种之一,具有广泛的应用价值。甘薯淀粉废水是不含有毒物质的高浓度有机废水,能够提供类球红细菌生长所需的营养物质。单因素实验表明,培养因素中甘薯淀粉废水的浓度、pH和温度为主要影响因子。正交试验优化后最佳培养条件为:甘薯淀粉废水稀释度60%,pH值7.0、温度35℃、接种量7.5%、转速200 r/min,此条件下,24 h类球红细菌浓度达6.7×109CFU/mL,与LB培养液(6.9×109CFU/mL)无显著差异,表明用甘薯淀粉废水培养类球红细菌是一种废水资源化利用的可行途径。
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| 关 键 词： | 甘薯淀粉废水 类球红细菌 正交设计 |
Optimization of Fermentation Conditions for Rhodobacter sphaeroides by Sweet Potato Starch Wastewater |
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| Institution: | Han Qingli et al. ( 1 Southwest Forestry University, Kuiming 650224, China ; 2 Research Center for Eco - Envirtmental Sciences, Chinese A- cademy of Seienses, Beijing 100085, China) |
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| Abstract: | As one of probiotics strains, Rhodobacter sphaeroides has extensive application value. Sweet potato starch wastewater is high concentration organic wastewater without toxic substances and can provides nutrients for Rhodobacter spha- eroides growth. Single factor experiment shows that the concentration, pH and temperature of sweet potato wastewater are the main influence factors. Orthogonal experiment shows the optimum culture conditions of Rhodobacter sphaeroides： the wastewater was diluted to 60%, pH 7.0, temperature 35℃, illumination 300 lx, inoculum 7.5%, rotate speed 200 r/min. After 24 h cultivation, Rhodobacter sphaeroides cells reached to 6.7 ×10^9 CFU/mL, which showed no significant difference comparing to LB culture medium. Result Indicates it is feasible to ferment Rhodobacter sphaeroides using sweet potato starch wastewater. |
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| Keywords: | Sweet potato starch wastewater Rhodobacter sphaeroides Orthogonal experiment |
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