以带蒂筋膜瓣为MGBR屏障膜的促成骨作用及作用方式

目的研究带蒂筋膜瓣为引导性骨再生技术屏障膜的促成骨作用及其作用方式．方法50只成年新西兰大白兔，雌雄不限，尺骨造2cm骨缺损模型，利用显微外科技术在骨缺损邻近制备一个带有无名血管蒂所属毛细血管网的筋膜瓣（带蒂筋膜瓣），使其包裹由自体骨髓基质干细胞与含骨形态发生蛋白的骨诱导活性材料构建的非细胞型组织工程骨并充填骨缺损，A...

Studies on Curative Effect and Osteogenesis in the Process of Bone Defect Repair by Pedicle Fasciai Flap as Membrane Material and GBR Technique

Objective To study the curative effect and osteogenesis in process of critical size bone defect repair using pedieal faseial flap with vessels as membrane material and bone regeneration technique. Methods 50 New Zealand rabbits with 5-month-old were used to prepare hibateral periosteum-including bone defect models in the middle of ulna and the length of defect was 2era. Autologous red bone marrow was implanted in osteoinductive absorbing material to prepare tissue engineering bone. The prepared uncellular tissue engineering compound was implanted in bone defect area and those whose defects were not wrapped by anything were divided into Group A while those whose defects were wrapped by pedical fascial flap with blood supply were divided into Group B. In weeks 4, 8, 12, and 16 after operation, rabbits in each group were examined by the radiograph （X-ray）, light density measurement, gross morphology and histological inspection, bone shape measurement was analyzed in junctional zone. In weeks 8, 12, 16 they were examined by biomechanics determination. The data were analyzed Statistically to check the difference of bone defect repair. Results The indexes in gross morphology and histological inspection, radiograph, bone shape measurement analysis, quantity and forming speed of bone trabecula and cartilagi nous tissue, formation of mature bone structure, remodeling of diaphysis, recanalization of cavum ossis and absorption and degradation of implant group of pedical fascial flap with blood supply, Group B was superior to that of Group A obviously. By comparision of light density measurement and biomechanics determination, the difference between two groups in weeks 12, 16 after operation and the difference of the same group at each time point all bad statistical significance （P d0.05）. In weeks 4, 8, 12, 16 after the operation the ratio between new bone trabecular area and total area had significant difference （ P〈 0.05）. Conclusion The pedical fascial flap with vessels can be used in guided bone regeneration technique. And it has obvious effect in promoting the process of bone defect repair.