‘富有’和‘次郎’甜柿叶片再生植株的研究

 比较了不同生长调节剂及其种类浓度和培养时间对‘富有’和‘次郎’两个甜柿(Diospyros kaki Thunb. ) 品种的叶片愈伤组织诱导和不定芽再生的影响。TDZ诱导叶片愈伤组织和不定芽再生的能力很强, 在含ZT 0.5 mg·L ^{- 1}的培养基中, 愈伤组织的形成率达96% , 愈伤组织不定芽分化率达80%以上,由0.5 mg·L ^{- 1} TDZ诱导形成的富有和次郎愈伤组织, 在含有ZT 0.5 mg·L ^{- 1}的1/2DKW培养基培养30 d,不定芽分化率均达96%以上, 平均芽数分别为7.8和5.4, 所得再生苗的生根率近75%。

Shoot Regeneration from Leaves of 'Fuyu' and 'Jiro' Persimmon

Studies were carried out on high-frequency shoot regeneration from leaves of Fuyu and Jiro,two Japanese non-astringent persimmon. The effects of growth regulator and cultural time on the adventitious shoot regeneration from leaves were investigated. The results showed that TDZ was a strong and efficient hormone inducing adventitious shoot and calli formation. The best regeneration was achieved in 1 /2DKW + TDZ 0.5 mg·L ^{- 1} + ZT 1.0 mg·L ^{- 1} , in which callus percentage was 96% and shooting was near above 80%.After 30 day culture, the calliwith small shootingwere transferred to 1 /2DKW medium supp lemented with 0.5 mg·L ^{- 1} zeatin and 0.1 mg·L ^{- 1} IAA. Shooting regeneration rate were more than 96% , and average adventitious shoots per exp lantwere 7.8 and 5.4 respectively for Fuyu and Jiro after 30 days culture. Rooting percentage from regenerated shootswas about 75%.