植物激素对附子中生物碱含量的影响

摘要：目的：研究植物激素对附子中乌头类总生物碱，新乌头碱，乌头碱和次乌头碱含量的影响。方法：在苗期和子根膨大期喷施不同的激素，采用酸性染料比色法测定乌头类总生物碱的含量，RP-HPLC测定新乌头碱，乌头碱和次乌头碱的含量。结果：6-BA和IAA两处理对乌头类总生物碱的含量有抑制作用，GA、KT、NAA作用不大；NAA提高了附子中新乌头碱和乌头碱的含量，降低了次乌头碱的含量；其它激素对三种生物碱的含量都有抑制作用。结论：激素对附子中生物碱的含量有影响。该试验结果为植物激素在附子栽培上的应用提供了科学依据。

Effects of Plant Hormones on the Content of Alkaloids in Aconitum carmichaeli

Study on the effects of plant hormones on the content of total alkaloids,mesaconitine,aconitine and hypaconitine in Aconitum carmichaeli.The hormones were sprayed on the Aconitum carmichaili at the seedling growing period and tubers expanding period.Acid dye colorimetry and RP-HPLC was used to the detection of the total alkaloids and mesaconitine,aconitine and hypaconitine.The results showed that the treatment of 6-BA and IAA had inhibitory effects on the content of total alkaloids,while GA,KT,NAA were not influence the result.NAA increased the content of mesaconitine,aconitine,but inhibited the hypaconitine.The other hormones inhibited the contents of the three alkaloids.The hormones had the effects on the content of alkaloids in Aconitum carmichaeli.The results can provide scientific basis for the application of plant hormones on Aconitum carmichaili during cultivation.