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Identification of a molecular marker linked to an Agropyron elongatum-derived gene Lr19 for leaf rust resistance in wheat |
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| Authors: | D P Cherukuri S K Gupta A Charpe S Koul K V Prabhu R B Singh Q M R Haq S V S Chauhan WE Weber |
| Institution: | Division of Genetics and National Phytotron Facility, Indian Agricultural Research Institute, New Delhi-110012, India;;Department of Pathology, University Medical Centre, University of Arizona, Tucson, AZ 85724, USA (Present address);;1291, Sector D, pocket I, Vasant kunj, New Delhi-110070, India;;Department of Biosciences, Jamia Millia Islamia, New Delhi-110025, India;;School of Life Sciences, Dr B. R. Ambedkar University, Agra, India; |
| Abstract: | The leaf rust resistance gene Lr19, transferred from Agropyron elongatum into wheat (Triticum aestivum L.) imparts resistance to all pathotypes of leaf rust (Puccinia recondita f.sp. tritici) in South‐east Asia. A segregating F2 population from a cross between the leaf rust resistant parent ‘HW 2046’ carrying Lr19 and a susceptible parent ‘Agra Local’ was screened in the phytotron against a virulent pathotype 77‐5 of leaf rust with the objective of identifying the molecular markers linked to Lr19. The gene was first tagged with a randomly amplified polymorphic DNA (RAPD) marker S73728. The RAPD marker linked to the gene Lr19 which mapped at 6.4 ± 0.035 cM distance, was converted to a sequence characterized amplified region (SCAR) marker. The SCAR marker (SCS73719) was specific to Lr19 and was not amplified in the near‐isogenic lines (NILs) carrying other equally effective alien genes Lr9, Lr28 and Lr32 enabling breeders to pyramid Lr19 with these genes. |
| Keywords: | Agropyron elongatum—Lr19—Puccinia recondita f sp tritici—Triticum aestivum— bulked segregant analysis — marker-assisted selection — RAPD marker — SCAR marker |