| 胶束纸色谱法分离和鉴定黄酮类化合物 |
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| 引用本文： | 佘戟,陈杰,莫丽儿,梁念慈.胶束纸色谱法分离和鉴定黄酮类化合物[J].湛江医学院学报,1999,17(2):102-104. |
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| 作者姓名： | 佘戟 陈杰 莫丽儿 梁念慈 |
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| 作者单位： | 广东医学院化学教研室 |
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| 基金项目： | 广东省高教厅重点学科专项基金,广东医学院青年科学基金 |
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| 摘 要： | 目的：探讨胶束纸色谱在分离，鉴定黄酮类化合物中的应用。方法：采用高于临界胶束浓度的ＳＤＳ，ＢｚＢｒ水溶液作展开剂通过纸色谱法分离和检测桑色素，杨梅黄素等８种黄酮甙和甙元，并对胶束浓度和有机添加剂影响分离效果作了观察。结果：０。．０１－０．０２ｍｏｌ／Ｌ的ＳＤＳ或ＢｚＢｒ胶束展开体系适宜于分离黄酮甙类，而当展开剂中０．０６ｍｏｌ／Ｌ的ＳＤＳ水溶液与乙酰乙酸乙酯的体积比为１０：１时，黄酮甙元可以得到满
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| 关 键 词： | 胶束纸色谱 黄酮类 添加剂 |
| 修稿时间： | 1998-08-10 |
Separation and identification of flavonoids with Micellar paper chromatography |
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| SheJi,ChenJie,MoLier,etal.Separation and identification of flavonoids with Micellar paper chromatography[J].Journal of Guangdong Medical College,1999,17(2):102-104. |
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| Authors: | SheJi ChenJie MoLier etal |
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| Abstract: | Objiective: ToinvestigatetheuseofMicellarpaperchromatography(MPC)ontheseparationandidentificationofflavonoids. Methods: Morin,Myricetin,Quercetin,Chrysin,Genistein,Hesperidin,NaringinandRutinwereseparatedandidentifiedbySDSorBzBrsolutionabovecriticalmicelleconcentrationonpaperchromatography.Theeffectsofthemicelleconcentrationandratiooforganicadditivewereinvestigated. Results: Toseparateflavoniedglycosides,thebettermicelleconcentrationofSDSorBzBris0.010.02mol/L;thespoteffectsofflavonoidaglyconesonMPCcanbeimprovedwhentheratioof0.06mol/LSDSsolutionandacetoaceticesteris10:1(v/v)inmobilephase. Conclusion: MPCisasimple,rapidandaccuratemothodtoanalyzeandseparateflavonoids. |
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| Keywords: | micellarpaperchromatography Flavonoids Additive |
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