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| 茶叶儿茶素对发根农杆菌的抑制作用及抗酚菌株筛选研究 | |
| 引用本文： | 毛清黎,施兆鹏,李玲,刘仲华,朱旗.茶叶儿茶素对发根农杆菌的抑制作用及抗酚菌株筛选研究[J].茶叶科学,2007,27(3):243-247. |
| 作者姓名： | 毛清黎 施兆鹏 李玲 刘仲华 朱旗 |
| 作者单位： | 1. 孝感学院生命科学学院,湖北,孝感,432100;教育部茶学重点实验室,湖南,长沙,410128 2. 教育部茶学重点实验室,湖南,长沙,410128 3. 华南师范大学生命科学学院,广东,广州,510631 |
| 基金项目： | 湖北省自然科学基金 , 湖北省教育厅科研项目 |
| 摘 要： | 本文研究了茶叶儿茶素对发根农杆菌的抑制作用并进行了抗酚菌株的筛选。结果表明:儿茶素(TC80)对发根农杆菌有明显的抑制作用,其MIC90为25～100μg/ml,这阐明了茶树中富含儿茶素等多酚类是制约农杆菌介导法对茶树进行遗传转化的关键因子。通过抗酚筛选获得的抗酚菌株R1000AP,其抗酚性提高10～40倍,其诱导茶树的发根频率提高46.5%(P<0.05)。 |
| 关 键 词： | 儿茶素 发根农杆菌 抑制作用 抗酚菌株 筛选 发根频率 |
| 文章编号： | 1000-369X（2007）03-243-05 |
| 收稿时间： | 2007-03-21 |
| 修稿时间： | 2007-03-212007-05-21 |
Study on Inhibitation of Agrobacterium rhizogenes by Tea Catechin and Screening of Anti-polyphenol Strain |
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| MAO Qing-li,SHI Zhao-peng,LI Lin,LIU Zhong-hua,ZHU Qi.Study on Inhibitation of Agrobacterium rhizogenes by Tea Catechin and Screening of Anti-polyphenol Strain[J].Journal of Tea Science,2007,27(3):243-247. | |
| Authors: | MAO Qing-li SHI Zhao-peng LI Lin LIU Zhong-hua ZHU Qi |
| Institution: | 1. College of Life Science,Xiaogan University, Xiaogan 432100, China; 2. Key Laboratory of Tea Science of National Ministry of Education, Changsha 410128, China; 3. College of Life Science, South China Normal University, Guangzhou 510631, China |
| Abstract: | This paper investigated inhibition of Agrobacterium rhizogenes by tea catechin and screening of the anti-polyphenol strain.The results showed that the catechin (TC80), which MIC90 was 25～100 μg/ml, showed the obvious inhibition of Agrobacterium rhizogenes. This showed that the plenty of polyphenols in the tea plant was a key factor to restrict Agrobacterium rhizogenes-mediated transformation of tea plant. An anti-polyphenol strain R1000AP obtained by anti-polyphenol screening, which anti-polyphenol capability enhanced 10～40 times. The hairy root frequency induced by the strain increased by 46.5%(P<0.05)compared to the non-anti-polyphenol strain. |
| Keywords: | catechin Agrobacterium rhizogenes inhibitatory action anti-polyphenol strain screen hairy root frequency |
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