烟草丛顶病伴随RNA临沧分离物基因组全长序列测定和结构分析 |
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引用本文: | 赵兴能,陈海如,莫笑晗.烟草丛顶病伴随RNA临沧分离物基因组全长序列测定和结构分析[J].安徽农业科学,2013(20):8480-8480,8524. |
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作者姓名: | 赵兴能 陈海如 莫笑晗 |
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作者单位: | 云南农业大学植物保护学院,云南昆明650201;云南省烟草农业科学研究院,云南昆明650031;云南农业大学植物保护学院,云南昆明,650201;云南省烟草农业科学研究院,云南昆明,650031 |
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基金项目: | 云南省科学技术厅项目(2011FB143);云南省烟草公司项目(2011YN36-1). |
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摘 要: | 目的]对烟草丛顶病伴随RNA(TBTDaRNA)临沧分离物基因组全长序列进行测定,并对其进行结构分析。方法]应用RT-PCR方法扩增烟草丛顶病伴随RNA临沧分离物的基因组,并进行全长序列的测定,同时对烟草丛顶病伴随RNA临沧分离物和龙陵分离物的基因组进行比较分析。结果]烟草丛顶病伴随RNA临沧分离物和龙陵分离物的基因组全长序列一致性为95.4%;ORF1a编码产物的氨基酸序列一致性为93.1%;ORF1b编码产物的氨基酸序列一致性为98.3%;3’端非编码区的核苷酸序列一致性为95.5%。结论]该研究为研究TBTDaRNA的分子变异奠定了基础。
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关 键 词: | 烟草丛顶病伴随RNA 基因组 序列测定 |
Complete Nucleotide Sequence and Genome Organization of A Lincang Isolate of Tobacco Bushy Top Disease-associated RNA |
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Institution: | ZHAO Xing-neng et al - ( College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650201 ) |
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Abstract: | Objeetive] The aim was to sequence the complete nueleotide and analyze the genome organization of a Lineang isolate of Tobacco bushy top disease-associated RNA (TBTDaRNA). Method] The full -length genome of a Lineang isolate of TBTDaRNA was amplified by RT- PCR and sequenced. Comparisons were carried out at the genomes level for TBTDaRNA Lineang isolate and Longling isolate. Result] The TBT- DaRNA Lineang isolate and Longling isolate had a 95.4% nueleotide identity in the full - length genomie sequence, and 93.1% and 98.3 % anli- no acid identity between their ORF1 a and ORF1 b, respeetively ; the nueleotide identity between their 3'untranslated regions was 95.5 %. Conclu- sion] The study laid a foundation for the research of molecular variability of TBTDaRNA. |
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Keywords: | Tobacco bushy top disease-associated RNA Genome Sequence |
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