| 甘草悬浮培养细胞与甘草草药、植株中甘草酸的检测 |
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| 引用本文： | 李楠,高广瑞,魏景芳,李冬杰,刘颖,薛志忠.甘草悬浮培养细胞与甘草草药、植株中甘草酸的检测[J].华北农学报,2007,22(B08):197-199. |
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| 作者姓名： | 李楠 高广瑞 魏景芳 李冬杰 刘颖 薛志忠 |
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| 作者单位： | [1]河北科技大学生命科学与工程学院,河北石家庄050018 [2]河北省科技厅,河北石家庄050021 |
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| 基金项目： | 河北省科技攻关项目（02245512D） |
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| 摘 要： | 采用不同的萃取剂从甘草的根、茎、叶、草药和甘草悬浮培养细胞中萃取甘草酸。结果表明,选择含氨0.5%、浓度为10%的乙醇溶液作为萃取剂,萃取得到的甘草酸粗品含量均高于50%乙醇萃取的萃取含量,检测出在优选甘草悬浮培养细胞中所含的甘草酸铵的含量高于其他材料,其中通过细胞悬浮培养,获得的甘草酸铵含量最高可达160.81 mg/g。
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| 关 键 词： | 甘草悬浮培养细胞 萃取剂 甘草酸 |
| 文章编号： | 1000-7091（2007）增刊-0197-03 |
| 修稿时间： | 2007-02-20 |
Detection of Glycyrrihizic Acid in Glycyrrihizic Suspension Culture Cell and Herbal Medicine, Plant |
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| LI Nan, GAO Guang-rui, WEI Jing-fang, LI Dong-jie, LIU Ying, XUE Zhi-zhong.Detection of Glycyrrihizic Acid in Glycyrrihizic Suspension Culture Cell and Herbal Medicine, Plant[J].Acta Agriculturae Boreali-Sinica,2007,22(B08):197-199. |
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| Authors: | LI Nan GAO Guang-rui WEI Jing-fang LI Dong-jie LIU Ying XUE Zhi-zhong |
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| Institution: | 1. College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China ;2. The Science and Technology Department of Hebei Province, Shijiazhuang 050021, China |
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| Abstract: | By different extractant, glycyrrihizic acid can be extracted from glycyrrihizic roots, stems, leaves, herbal medicine and Glycyrrihizic suspension culture cells. The crude content of glycyrrihizic acid extracted by 10 % ethanol containing 0,5 % ammonia was higher than that extracted by 50 % ethanol. The results showed the content of inammonium glycyrrhetate optimized glycyrrihizic suspension culture cells were higher than that in other materials. And also by cell suspension culture, the maximum content of glycyrrihizic acid can reach 160.81 mg/g. |
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| Keywords: | Glycyrrihizic suspension culture cell Extractant Glycyrrihizic acid |
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