山东灰霉病菌对腐霉利的抗药性检测

为监测灰霉病菌对腐霉利的抗药性，2012 年12 月到2013 年5 月，在山东省内10 个市县，采集灰霉病菌样本150 余份，分离得到灰葡萄孢(Botrytis cinerea)菌株130 株，采用生长速率法对130 株灰霉病菌进行抗药性检测。结果表明，腐霉利对灰霉病菌的EC50的范围为0.1208~12.486 μg/mL，平均EC50为4.03 μg/mL，抗性频率达到67.69%。其中低抗菌株占52.38%，中抗菌株占19.048%，未检测到高抗菌株。本研究可为灰霉病的防治特别是杀菌剂的轮换使用提供依据。

Resistance Detection for Botrytis cinerea Against Procymidone in Shandong Province

In order to monitor procymidone resistance of Botrytis cinerea, from December of 2012 to May of 2013, many Botrytiscinereasamples were collected from 10 cities in Shandong, and 130 isolates were purified from them. The resistances of Botrytis cinerea were determined in 130 isolates by measuring the mycelia growth on the fungicide-amended media. The means of the 50% effective concentration values （EC50） were 4.03 μg/mL. The resistance frequency was 67.69%, 52.38% of Botrytis cinerea was low resistant strains, 19.048% of Botrytis cinerea was moderate resistant strains; there was no high resistant strain. The results provided a theoretical basis for using fungicides efficiently to prevent and control botrytiscinereain the field.