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| 野菊花蕾的组培与快繁技术研究 | |
| 引用本文： | 韩磊,吕鑫,张文静,杨小霁. 野菊花蕾的组培与快繁技术研究[J]. 北方园艺, 2009, 0(5) |
| 作者姓名： | 韩磊 吕鑫 张文静 杨小霁 |
| 作者单位： | 潍坊职业学院,山东,潍坊,261041 |
| 摘 要： | 利用组织培养的方法,首次以野菊花蕾作为外植体,借鉴前人组织培养观赏菊花的经验,找到一种使野菊能够快速增殖的方法.以MS为基本培养基,添加BA 1.0 mg/L和NAA0.2 mg/L作为外植体的分化与继代培养基,得到愈伤组织和大量的丛生芽;用MS培养基进行增殖培养;用1/2MS+BA 1.0 mg/L+NAA 0.2 mg/L进行生根培养,生根率在98%以上.最后通过驯化练苗得到大量的野菊再生植株. |
| 关 键 词： | 野菊 花蕾 组织培养 |
Study on Flower Bud Tissue Culture and Rapid Propagating Technique of Chrysanthemum indicum L. |
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| HAN Lei,LV Xin,ZHANG Wen-jing,YANG Xiao-ji. Study on Flower Bud Tissue Culture and Rapid Propagating Technique of Chrysanthemum indicum L.[J]. Northern Horticulture, 2009, 0(5) | |
| Authors: | HAN Lei LV Xin ZHANG Wen-jing YANG Xiao-ji |
| Affiliation: | Weifang Vocational College;Weifang;Shandong 261041;China |
| Abstract: | Flower bud of Chrysanthemum indicum L.as explant was studied for rapid propagation through tissue culture for the first time in the light of ornamental Chrysanthemum experiences put forward in the past.Calli and multiple shoots was obtained by MS basic medium supplemented with 1.0 mg/L BA and 0.2 mg/L NAA as differentiation and subculture media.Multiplication culture was on the MS medium.Shooting culture was on 1/2 MS medium supplemented with 1.0 mg/L BA and 0.2 mg/L NAA.The rooting ration was above 98%.At ... |
| Keywords: | Chrysanthemum indicum L Flower bud Tissue culture |
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