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NaCl胁迫下Si对甘草叶片抗氧化酶活性的影响
引用本文:崔佳佳,张新慧,张恩和,李月彤,周达.NaCl胁迫下Si对甘草叶片抗氧化酶活性的影响[J].干旱地区农业研究,2017,35(3):172-177.
作者姓名:崔佳佳  张新慧  张恩和  李月彤  周达
作者单位:1. 甘肃农业大学农学院,甘肃兰州,730070;2. 宁夏医科大学药学院,宁夏银川750004;宁夏回药现代化工程技术研究中心,宁夏银川750004;宁夏回医药协同创新中心,宁夏银川750004;宁夏医科大学回医药现代化省部共建教育部重点实验室,宁夏银川750004;3. 宁夏医科大学药学院,宁夏银川,750004
摘    要:为探究NaCl胁迫下Si对甘草抗逆生理特性的影响,通过盆栽试验,研究不同浓度Si对甘草叶片抗氧化酶活性(SOD、POD、CAT)、MDA含量、质膜相对透性以及可溶性蛋白和脯氨酸含量的影响。结果表明,Si2(0.2g·kg-1)处理为最适浓度,可显著提高甘草叶片SOD、POD、CAT活性,与对照(Si0)相比,这3种酶活性在Si2处理下分别增加37.44%、26.98%、35.16%;Si3(0.6 g·kg-1)处理显著降低膜脂过氧化物MDA含量;增加质膜相对透性、可溶性蛋白含量;降低脯氨酸含量,MDA含量降低35.63%;质膜相对透性增加17.69%;可溶性蛋白含量增加36.62%;脯氨酸含量降低38.15%。由此可见,Si可以通过提高抗氧化酶活性来减轻活性氧及自由基对甘草叶片的毒害作用,即加Si可改善甘草在NaCl胁迫中的抗逆特性,缓解了NaCl胁迫对甘草的伤害。

关 键 词:甘草  Si  NaCl胁迫  抗氧化酶

Effect of silicon addition on the antioxidant enzymes activities of licorice leaves under salt stress
CUI Jia-ji,ZHANG Xin-hui,ZHANG En-he,LI Yue-tong,ZHOU Da.Effect of silicon addition on the antioxidant enzymes activities of licorice leaves under salt stress[J].Agricultural Research in the Arid Areas,2017,35(3):172-177.
Authors:CUI Jia-ji  ZHANG Xin-hui  ZHANG En-he  LI Yue-tong  ZHOU Da
Abstract:The experiment was carried out to study the effects of silicon on antioxidant enzymes(SOD,POD,and CAT) and osmolytes content of licorice leaves under NaC1 stress by pot experiment.The results showed that the concentration of 0.2 g· kg-1 silicon had the strongest effect increasing the activities of SOD,POD,and CAT,by 37.44%,26.98%,and 35.16% compared with the control,respectively.For the concentration of 0.6 g· kg-1,MDA was decreased by 35.63%,proline content by 38.15%,while the relative membrane permeability was increased by 17.69%,and the soluble protein content by 36.62%.In conclusion,silicon treatment could alleviate the damage degree of NaC1 stress by promoting the antioxidant enzymes activities in licorice leaves.
Keywords:licorice  silicon  salt stress  antioxidant enzyme
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