Chelex-100快速提取动物源饲料DNA方法的建立

为防止疯牛病疫区反刍动物源性饲料传入我国,非常有必要对贸易往来中的肉骨粉品种来源进行鉴定。本文建立了一种利用Chelex—100快速提取动物源性饲料DNA的方法,用于反刍动物源性饲料的PCR扩增分析。结果显示该方法与GuSCN法、SDS法同样理想,从而实现了对进境饲料的快速检定的要求。该方法检测灵敏度达到牛成分含量为0．1％的水平,具有简单、快捷、灵敏度高的优点。

The Application of Chelex-100 Method to Extract the Genomic DNA of the Animal Derived Feedstuff

To forbid the production, circulation and use of animal derived feedstuffs from the epidemic region is an important measure for control of infection and epidemic of Bovine spongiferm encephalopathy(BSE). A PCR method has been established to check any contamination of ruminant derived feedstuff using, Chelex-100 extracted DNA template. According to the result of PCR, the effects that templates were prepared using the "Chelex method" and the usual method were the same. But the cross contamination and DNA waste were reduced in the "Chelex method". The lowest limit of ruminant derived feedstuff of this method is 0.1 %. The determination method used in this study is simple, quick and rather reliable.