豫北及冀南地区4个小麦禾谷孢囊线虫群体的种类鉴定

禾谷孢囊线虫病(Cereal Cyst Nematode,CCN)是世界上为害小麦等禾谷类作物的重要根部线虫病害,许多国家受害严重1].该病的病原禾谷孢囊线虫(Heterodera avenae group)是一个复合种群组,包括12个有效种和几个未命名的种,其中发生普遍、危害严重的有燕麦孢囊线虫(H.avenae)、菲利普孢囊线虫(H.filipjevi)、大麦孢囊线虫(H.hordecalis)和麦类孢囊线虫(H.latipons)1].我国1987年在湖北省天门县首次在小麦上发现禾谷孢囊线虫病,Wang等2]鉴定病原为燕麦孢囊线虫(H.avenae);后陆续在河南、安徽、山东等多个省份发现该病,病原均为该种线虫.2010年Li等3]在河南许昌报道发现了菲利普孢囊线虫(H.filipjevi).

Identification on the species of cereal cyst nematode from Northern Henan and Southern Hebei,China

Four cereal cyst nematode(CCN) populations of wheat were collected from Boai,Qingfeng,Anyang counties in Henan Province and Handan county in Hebei Province in 2009.The cyst of Boai population had distinct under-bridge in the vulval cone area,but other three tested populations had not.The main morphological characteristics and measurements of the cyst and the second juvenile of Boai population conformed to Heterodera filipjevi,and that of the populations from Qingfeng,Anyang and Handan conformed to H.avenae.The result of ITS sequence analysis and alignment indicated that Boai population and related species H.filipjevi from America and Italy were clustered in the same group with close relationship by MP of MEGA 4,and the three populations from Qingfeng,Anyang,Handan and the related species H.avenae from Tongzhou and Zhengzhou were clustered in the same group.Based on the morphological and molecular characteristics,the Boai population was identified as H.filipjevi and the other three populations were identified as H.avenae.