Genetic variability and population structure of <Emphasis Type="Italic">Grapevine virus A</Emphasis> coat protein gene from naturally infected Italian vines |
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Authors: | Sergio Murolo Gianfranco Romanazzi Adib Rowhani Angelantonio Minafra Pierfederico La Notte Maria Barbara Branzanti Vito Savino |
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Institution: | (1) Dipartimento di Scienze Ambientali e delle Produzioni Vegetali, Universitá Politecnica delle Marche, Via Brecce Bianche, Ancona, 60131, Italy;(2) Department of Plant Pathology, University of California, Davis, CA, USA;(3) Dipartimento di Protezione delle Piante e Microbiologia Applicata, Universitá degli Studi di Bari e Istituto di Virologia Vegetale, CNR Bari, Via Amendola 165/A, 70126 Bari, Italy |
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Abstract: | Grapevine virus A (GVA) is considered one of the viruses associated with rugose wood (RW), one of the most economically important diseases
of grapevine. Thirty-seven GVA isolates collected from grapevine cultivars from Marche (central-eastern Italy), Apulia and
Campania (southern Italy), were subjected to molecular characterization. The genetic and population diversity was studied
in the coat protein (CP) gene by RT-PCR-RFLP analysis with three restriction enzymes (MseI, AluI, and AciI), and nucleotide sequencing. A new primer pair (CP1F/R) allowing amplification of the whole CP gene (621 bp) was developed.
RFLP with AciI yielded the highest number of variants in GVA isolates, showing seven different ‘simple’ profiles (A, B, C, D, E, F, and
G). ‘Complex’ profiles were also found, and the most common variant combination was A + B in 39% of isolates. The analysis
of GVA sequences confirmed the presence of plants infected with more than one GVA variant and suggested that RT-PCR-RFLP is
suitable for evaluating population diversity of GVA enabling a screening of different haplotypes. The distribution of RFLP
profiles and the phylogenetic analysis were not correlated with the location of infected plants, showing the presence of a
GVA population with genetic diversity in the average with those of RNA viruses. |
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Keywords: | Cloning and sequencing Diagnosis Primer RT-PCR-RFLP Vitis vinifera |
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