A tetraploid hybrid plant from 4x × 2x crosses in Vitis and its origin |
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Authors: | Sung Min Park Akira Wakana Michikazu Hiramatsu Kenji Uresino |
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Institution: | (1) Faculty of Agriculture, Kyushu University, Kasuya-gun 811-2307, Japan;(2) University Farm, Faculty of Agriculture, Kyushu University, Kasuya-gun 811-2307, Japan |
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Abstract: | To study the origin of unreduced (2n) gametes in diploid Vitis cultivars, we surveyed the occurrence of tetraploid hybrid seedlings from 40 interploid crosses with five tetraploid and
seven diploid cultivars. A total of 250 seedlings from the interploid crosses were established through embryo culture and
by seed sowing. In 20 2x × 4x crosses, no tetraploid hybrid seedlings were derived from 8,902pollinations. In 20 4x × 2x crosses,
two tetraploid hybrid seedlings were obtained from 8,057 pollinations. Investigation of isozyme genotypes of the two tetraploid
seedlings using three variable enzyme systems indicated that one of the two seedlings resulted from the union of a diploid
egg with a 2n male gamete and that failure of second meiotic division resulted in the formation of the 2n male gamete. The
percentage of giant pollen grains in `Muscat Bailey A', a pollen parent of the tetraploid seedling, was relatively high(about
5.9%) and 10.9% of the giant pollen grains germinated on agar medium. These results suggested that 2n pollen of diploid cultivars
are useful for breeding tetraploid grape.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | 2n pollen GPI-2 and PGM-2 isozymes interploid cross pollengermination unreduced male gamete Vitis complex |
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