Effect of donor plant age and inflorescence age on microspore culture of Brassica napus L. |
| |
Authors: | Y Takahata D C W Brown W A Keller |
| |
Institution: | (1) Faculty of Agriculture, Iwate University, 020 Morioka, Japan;(2) Plant Research Centre, Agriculture Canada, K1A 0C6 Ottawa, Canada;(3) Plant Biotechnology Institute, National Research Council, S7N 0W9 Saskatoon, Canada |
| |
Abstract: | Summary Effect of age of donor plants and age of inflorescence on embryogenesis in microspore culture of B. napus was examined. Microspores isolated from buds of older plants had a higher embryo yield than those of younger ones. The effect of the age of inflorescence showed a different pattern. In older plants, a higher embryogenesis response was observed in microspores isolated from buds of new inflorescences, while in young plants, microspores isolated from buds of old inflorescences showed high embryo yield. These different responses were considered to be attributable to a difference in the developmental stage of pollen at the time of microspore isolation. Our results indicated that microspores collected from older inflorescences and older plants have sufficient embryogenic potential when the optimum developmental stage of pollen was used. Frequency of embryo to plant conversion was influenced by the size of embryos subcultured, but not by donor plant age or the age of the inflorescence. |
| |
Keywords: | Brassica napus donor plant age embryogenesis haploid microspore culture microspore stage |
本文献已被 SpringerLink 等数据库收录! |
|