| 新疆哈密瓜细菌性斑点病离体叶片种子带菌检测及致病力分化 |
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| 引用本文： | 王爱英,李国英,曾义,张昕.新疆哈密瓜细菌性斑点病离体叶片种子带菌检测及致病力分化[J].西北农业学报,2007,16(4):216-219,222. |
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| 作者姓名： | 王爱英 李国英 曾义 张昕 |
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| 作者单位： | 1. 石河子大学绿洲农业生态重点实验室,新疆石河子,832003
2. 石河子大学农学院,新疆石河子,832003 |
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| 摘 要： | 应用甜瓜离体叶片检测甜瓜种子的带菌情况，分别采用不同的缓冲液和不同的接菌方法进行检测，发现PBＳ缓冲液富集病原菌效果好，注射接种效率高。同时，离体叶片是菌株致病力分化初步检测较好的材料，从田间分离所得菌株来看，黄瓜细菌性角斑和西瓜果腐菌株致病力均被分化为强、中、弱，而且从种子上分离的病原菌的致病力弱。
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| 关 键 词： | 哈密瓜 离体叶片 种子检测 致病力分化 |
| 文章编号： | 1004-1389（2007）04-0216-04 |
| 收稿时间： | 2006-05-31 |
| 修稿时间： | 2006-05-312007-03-05 |
Detection of Acidovorax in Melon Seed and Pathogenic Differentiation Using in Vitro Leaf in Xinjiang |
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| WANG Ai-ying,LI Guo-ying,ZENG Yi and ZHANG Xin.Detection of Acidovorax in Melon Seed and Pathogenic Differentiation Using in Vitro Leaf in Xinjiang[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(4):216-219,222. |
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| Authors: | WANG Ai-ying LI Guo-ying ZENG Yi and ZHANG Xin |
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| Institution: | Key Laboratory of Oasis Ecology Agriculture of Xinjiang Bingtuan, Shihezi University, Shihezi Xinjiang 832003, China;Agriculture Department Shihezi University, Shihezi Xinjiang 832003, China;Agriculture Department Shihezi University, Shihezi Xinjiang 832003, China;Agriculture Department Shihezi University, Shihezi Xinjiang 832003, China |
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| Abstract: | The different buffer solution separately examination the ratio of melon seed''s disease-carrying using leaf in vitro was conducted. The result showed that PBS buffer solution which had better effect to enrich bacteria.And the injection vaccination efficiency is high. The leaf is better material in vitro for pathogenic differentiation. Pseuodomonas syringae pv. Lachryman and Acidovorax avenae subsp.citrulare are compartmentalized as strong,middle and weak in pathogenic differentiation,disease germ''s pathogenesis strength is weak separated from the seed. |
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| Keywords: | Melon Leaf in vitro Detection in melon seed Pathogenic differentiation |
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