LAS、Cd~(2+)污染对鲫鱼鳃、肝脏SOD、Na~+-K~+-ATPase活性的影响

以鲫鱼为实验对象,采用不同质量浓度的LAS和Cd2+进行暴露实验,研究亚急性条件下,LAS、Cd2+及其复合污染对鲫鱼鳃、肝脏SOD、Na+-K+-ATPase活性的影响.结果表明:在单一LAS、Cd2+处理条件下,随LAS、Cd2+质量浓度增加,鲫鱼鳃、肝脏的SOD、Na+-K+-ATPase活性与对照组相比均呈浓度-效应关系;复合处理下,污染物质量浓度的不同组合对鲫鱼鳃、肝脏的SOD、Na+-K+-ATPase活性影响不同,其中当Cd2+质量浓度为0.4mg.L-1时,肝脏的SOD、Na+-K+-ATPase活性均随LAS质量浓度的增加而降低,各复合组的SOD、Na+-K+-ATPase活性均显著低于相应质量浓度的单一Cd2+处理组(P<0.05);2.0 mg.L-1Cd2+与5.0 mg.L-1LAS复合组鲫鱼鳃SOD、Na+-K+-ATPase活性、肝脏SOD活性显著低于相应质量浓度的单一Cd2+、LAS处理组(P<0.05).在一定质量浓度的组合下,LAS与Cd2+复合污染在SOD、Na+-K+-ATPase水平上表现为一定的协同作用.

Effects of combined LAS,Cd~(2+) pollution on the activities of SOD and Na~+-K~+-ATPase in the gill and liver of Carassius auratus

The effects of LAS,Cd2+ alone and combined pollution on the activities of SOD and Na+-K+-ATPase in the gill and liver tissues of Carassius auratus stressed for 14 days were studied in concentrations of LAS(1.0 mg·L-1,5.0 mg·L-1) and Cd2+(0.4 mg·L-1,2.0 mg·L-1) by using exposed experiment.The results showed that,compared with control,the dose-effect was observed under alone pollution both in the liver and gill.Different pollution combined treatments produced different effects in changes of two enzymes activities in the liver and gill.Thus,0.4 mg·L-1 Cd2+ induced the SOD and Na+-K+-ATPase activities decreased with the increase of LAS concentration,and the activities of two enzymes in the liver were lower under combined stress than that of Cd2+ treatment alone significantly(P<0.05),while 2.0 mg·L-1 Cd2+ and 5.0 mg·L-1 LAS induced the SOD and Na+-K+-ATPase activities in the gill and the SOD activity in the liver decreased significantly(P<0.05) compared with the Cd2+ or LAS treatment alone.It can be concluded that the joint effects of the combined pollution on the SOD and Na+-K+-ATPase activities were synergistic under certain LAS and Cd2+ concentrations.