外源脱落酸对铝胁迫下栝楼叶绿素荧光及生理活性的影响

通过比较不同栝楼品种的耐铝性差异,确定最佳缓解浓度,选取耐铝性有差异的保定栝楼和浦阳栝楼为试验材料,设置6组不同浓度的Al~(3+)和脱落酸,研究Al~(3+)和脱落酸的互作对2个栝楼品种植株的根长、叶绿素荧光特性、根系活力、MDA含量、抗氧化酶活性和抗氧化剂的含量。结果表明:(1)保定栝楼和浦阳栝楼在铝胁迫和外源脱落酸的作用下,各指标体现出相似的变化趋势,但是变化程度和敏感性显露出了一定的区别,保定栝楼具有更强的耐铝能力。(2)铝胁迫使得2个品种栝楼的根长、根系活力、叶绿素荧光参数中的F_v/F_m、F_m和ETR、抗氧化酶SOD、POD、CAT以及抗坏血酸-谷胱甘肽(AsA-GSH)循环系统中AsA、GSH含量都有所抑制,而F_0、MDA和DAsA上升。(3)外源脱落酸加入后,2个品种的抗氧化酶以及AsA、GSH均显现了先上升后下降的趋势,MDA持续降低,F_0和DAsA呈现出由降而升的趋势,其中5mg/L的外源脱落酸使栝楼的根长、根系活力、F_v/F_m、F_m、ETR、SOD、POD、CAT以及AsA、GSH上升到峰值,MDA下降,F_0和DAsA降到谷值。(4)经过隶属函数和耐铝系数的分析,2个品种都能得出相同的结果,铝胁迫下各浓度外源脱落酸对2个品种的缓解程度为5 mg/L7.5 mg/L2.5 mg/L0mg/L10mg/L。保定栝楼的耐铝能力强于浦阳栝楼,低浓度的外源脱落酸可以缓解铝胁迫,5 mg/L浓度的外源脱落酸缓解铝胁迫效果最佳,在生产实际中要选择耐铝性较强的栝楼进行栽培,并辅以适当浓度的外源脱落酸。

Effect of Exogenous Abscisic Acid on Chlorophyll Fluorescence and Physiological Activity of Trichosanthes kirilowii Maxim. Under Aluminum Stress

In order to improve the production of Trichosanthes kirilowii in the red soil and provide theoretical support for the cultivation of acid soils.Mainly in compare the difference of aluminum tolerance in between two places of origin,and determine the best relief concentration.Two kinds of Trichosanthes kirilowii Maxim.,Baoding Trichosanthes kirilowii and Puyang Trichosanthes kirilowiiis selected as the experiment materials.Six different concentrations of Ala + and abscisic acid were set up.To study the interaction of AP+ and ABA on the root length,chlorophyll fluorescence characteristics,root activity,MDA content,antioxidant enzyme activity and antioxidant content of two species of the plant.The results show:(1) Baoding Trichosanthes kirilowii and Pu Yang Trichosanthes kirilowii under the effect of aluminum stress and exogenous abscisic acid,each index reflects similar trend,but varying degrees of each index and sensitivity revealed some differences,Baoding Trichosanthes kirilowii has stronger resistance ability of aluminum.(2) Aluminum threat forced two varieties of Trichosantheskirilowii root length,root activity,Fv/Fm,Fmm,ETR,SOD,POD,CAT and ASA,GSH content are decreased,and F0,MDA and DAsA rise.(3) After exogenous abscisic acid is added,two varieties of Trichosanthes kirilowii root length,root activity,chlorophyll fluorescence parameters except F0,anti-oxidase and ascorbate glutathione cycle system (ASA and GSH) show the trend of first increased and then decreased,MDA was decreased continuously,F0 and DAsA presented by the descending and ascending trend.5 mg/L of exogenous abscisic acid make root length,root activity,Fv/Fm,Fmm,ETR,SOD POD,CAT and ASA,GSH rose to a peak,MDA decreased,F0and DAsA down to the valley.(4)Through membership function and the analysis of the aluminum resistance coefficient,two varieties of Trichosanthes kirilowii can get the same result,the ability of Al tolerance:5 mg/L＞7.5 mg/L＞2.5 mg/L＞0mg/L＞10mg/L.So the ability of aluminum resistance Baoding Trichosanthes kirilowii is stronger than Puyang Trichosanthes kirilowii,low concentration of exogenous abscisic acid can alleviate the stress of aluminum,but when the exogenous abscisic acid concentration is too high,unable to protect the plant from aluminum stress influence function,namely promoting at the low concentration and high concentration inhibit,5 mg/L ABA can effectively relieve Al stress,So we should select aluminum resistant Trichosanthes kirilowii to plant,supplemented with appropriate concentration of exogenous ABA.