基于紫外分光光度计技术测定谷子种子活力

为了准确评价谷子的种子活力,探索紫外分光光度计法用于谷子种子活力检测的可行性,提供一种快速、无损检测谷子种子活力的新方法。分别以2012、2013、2014、2015年收获,经自然风干后的‘豫谷18’的种子为材料,对谷子种子进行不同时间浸泡,根据浸出液的吸光值评价种子活力。结果表明,在28℃条件下,种子的发芽势与发芽率随着储藏年份的延长而降低,储藏年份越短,发芽率越高;清水浸泡相同储藏年份的谷子种子,其种子浸出液随着浸泡时间的延长OD值增大;不同储藏年份种子,OD值均随储藏年份的延长而增加;在220 nm波长下紫外吸光值与谷子标准发芽率存在极显著负相关。综合分析表明,在种子浸泡8 h后,在220 nm波长下OD值能客观评价谷子种子活力,为谷子种子活力的快速无损检测提供简便可操作的方法。

Seeds Vigor of Foxtail Millet: Determined by Ultraviolet Spectrophotometer

The aims are to evaluate the seed vigor of foxtail millet, study the feasibility of the UV spectrophotometer method used in seed vigor detection of foxtail millet, and provide a new method for rapid and non-destructive detection of seed vigor of foxtail millet. The naturally dried seeds of ‘Yugu 18’ from 2012, 2013, 2014 and 2015 were soaked for different time, and the seed vigor was evaluated according to the UV absorption value of the leaching solution. The results showed that the germination potential and germination rate of seeds at 28℃ decreased with the prolongation of the seed storage time. The shorter the seed storage time, the higher the seed germination rate was. After the foxtail millet seeds with the same storage time were soaked by water, its OD values of the leaching solution increased along with the prolonging soaking time. The OD values of the leaching solution of different-storage-year seeds increased along with the prolonging soaking time. The UV absorption value of the leaching solution at 220 nm was significantly and negatively correlated with the standard germination rate of the seeds. The comprehensive analysis showed that the OD value of seed after soaking for 8 h at 220 nm could objectively evaluate the viability of the seeds, and provide a simple and operational method for the rapid, non-destructive detection of seed viability.