150亿孢子/g球孢白僵菌可湿性粉剂的研发及对西花蓟马的防治应用

为了研发靶标害虫为西花蓟马的球孢白僵菌杀虫剂，我们首先通过筛选、纯化和复壮获得对西花蓟马高效的真菌菌株；其次，对液-固双相发酵条件和发酵工艺进行优化；然后，改良了产品剂型和配方；最后对产品进行环境和毒性评价。结果显示该产品各项指标均符合国家标准。150亿孢子/g球孢白僵菌可湿性粉剂于2018年在国内获审批登记。喷施1800～3000 g/hm²对辣椒上西花蓟马的防效均为74%以上。推荐用量为2400 g/hm²。该产品的研发和获批登记为西花蓟马的生物防治奠定了坚实的基础。

Research and Development of Wettable Powder of Beauveria bassiana and Its Control and Application to Frankliniella occidentalis

In order to develop an insecticide of Beauveria bassiana against Western Flower Thrips (WFT), Frankliniella occidentalis, firstly, fungal strains with high efficiency to WFT were selected by screening, purification and rejuvenation; secondly, the liquid-solid two-phase fermentation conditions and fermentation process were optimized; thirdly, the product formulation and formula were improved; fourthly, environment and toxicity assessment of the product were evaluated. The results showed that all the indicators of the product were in line with national standards. The 15 billion conidia/g B. bassiana wettable powder was approved and registered in China in 2018. The control efficacies of spraying 1800―3000 g/ha on thrips on pepper are above 74%. The recommended dosage is 2400 g/ha. The development and registration of the product have laid a solid foundation for the biological control of WFT.