明胶骨原料碳、氮稳定同位素的地域性差异研究

为探讨不同地域来源的明胶骨原料中碳、氮稳定同位素的分布特征及其相关性,本试验采用元素分析-稳定同位素比率质谱仪测定了广西、内蒙古和山东3个主产区的牛骨样品及当地牧草饲料样品中的¹³C和¹⁵N同位素组成,并结合线性回归讨论了牛骨样品中δ¹³C和δ¹⁵N值的相关性与牛食谱组成的关系。结果表明,广西地区骨样品中δ¹³C和δ¹⁵N值的相关性较好(R²=0.401 8),而内蒙古和山东地区骨样品中δ¹³C和δ¹⁵N值的相关性较差(R²<0.1),反映了3地牛食物的来源稳定性及主要成分存在明显差异,对各地牛食谱结构推测的结果与实际牧草饲料样品中δ¹³C和δ¹⁵N值的地区分布规律也较为一致。本研究初步揭示了明胶骨原料中碳、氮稳定同位素产生地域性差异的原因,不仅为动物生态学研究提供了有益信息,更为动物制品产地溯源研究提供了新思路。

Regional Differences of Carbon and Nitrogen Stable Isotopes in Bone Raw Materials for Gelatin Production

In order to analyze the regional distribution characteristics of the carbon and nitrogen stable isotopes in bone raw materials for gelatin production, C and N isotopic compositions in bovine bone samples and forage samples from three different provinces (including Guangxi, Inner Mongolia and Shandong) were determined using elemental analyzer-isotope ratio mass spectrometry. The relationship between the correlation on δ¹³C and δ¹⁵N in bovine bone samples and local cattle’s diet composition were also discussed by linear regression analysis. The results showed that δ¹³C and δ¹⁵N in bone samples from Guangxi correlated well (R²=0.401 8), while the correlation on δ¹³C and δ¹⁵N in bone samples from Inner Mongolia and Shandong was poor (R² < 0.1), indicating that the stability and main components of local cattle’s diet were obviously different among the three regions. Moreover, the conjectures of local cattle’s diet composition were consistent with the regional distribution characteristic of δ¹³C and δ¹⁵N in actual forage samples. This study preliminarzy revealed the reasons for the regional differences of carbon and nitrogen stable isotopes in bone raw materials, which provides useful information for animal ecology research and a new idea for geographical origin traceability of animal products.