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灵武长枣多糖含量测定的研究
引用本文:杨军,章英才,苏伟东,杨淑娟.灵武长枣多糖含量测定的研究[J].北方园艺,2011(14):35-37.
作者姓名:杨军  章英才  苏伟东  杨淑娟
作者单位:宁夏大学生命科学学院,宁夏,银川,750021;宁夏红枣工程技术研究中心,宁夏,灵武,750400
基金项目:宁夏回族自治区自然科学基金资助项目(NZ1014)
摘    要:灵武长枣样品经脱脂,脱小分子糖后,用蒸馏水沸水浴浸提、醇沉、洗涤得灵武长枣多糖,含量测定采用苯酚-硫酸比色法,用精制多糖测得其多糖对葡萄糖的换算因子。建立了一种简便、准确测定灵武长枣多糖含量的方法。结果表明:该方法测定简便可行,重现性较好,平均回收率为97.11%,所测全红灵武长枣样品中多糖含量为13.30%。

关 键 词:灵武长枣  多糖  苯酚-硫酸比色法  含量测定

Isolation and Assaying of Polysaccharide in Zizyphus jujuba Mill cv.lingwuchangzao
YANG Jun,SU Wei-dong,YANG Shu-juan,ZHANG Ying-cai.Isolation and Assaying of Polysaccharide in Zizyphus jujuba Mill cv.lingwuchangzao[J].Northern Horticulture,2011(14):35-37.
Authors:YANG Jun  SU Wei-dong  YANG Shu-juan  ZHANG Ying-cai
Institution:YANG Jun1,SU Wei-dong2,YANG Shu-juan1,ZHANG Ying-cai1(1.College of Life Science,Ningxia University,Yinchuan,Ningxia 750021,2.Ningxia Jujube Engineering Technology Research Center,Ningxia 750021)
Abstract:With ungrease treatment and oligo-saccharide,and extract by boiling water-bath,ethanol precipitation,deprivate protein,and determinated its content by phenol-sulfuric acid method.This experiment established a simple,accurate determination method of polysaccharide form Zizyphus jujuba Mill cv.lingwuchangzao.The results showed that it was a simple,feasible method to determinate polysaccharide content,the recovery was 97.11%,the polysaccharide in Zizyphus jujuba Mill cv.lingwuchangzao was 13.30%.
Keywords:Zizyphus jujuba Mill cv  lingwuchangzao  polysaccharide  phenol-sulfuric acid colorimetry  determination  
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