| 西瓜不定芽分化过程中同工酶的变化研究 |
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| 引用本文： | 张丽珍,杨冬业,秦新民.西瓜不定芽分化过程中同工酶的变化研究[J].北方园艺,2011(14):108-110. |
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| 作者姓名： | 张丽珍 杨冬业 秦新民 |
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| 作者单位： | 桂林师范高等专科学校,广西,桂林,541001;桂林医学院,广西,桂林,541001;广西师范大学生命科学学院,广西,桂林,541004 |
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| 基金项目： | 广西壮族自治区“药用植物学概论”精品课程资助项目 |
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| 摘 要： | 采用聚丙烯酰胺凝胶电泳(PAGE)结合同工酶染色的方法对华玲西瓜不定芽分化过程中过氧化物酶(POD)同工酶、酯酶(EST)同工酶进行分析。结果表明:在不定芽分化过程中,过氧化物酶同工酶变化较大,第20天的酶含量达到峰值,总体上分化后期比前期出现的酶带多且含量大,酶活力高;酯酶同工酶变化不大,酶带数后期比前期较多,但酶带的含量后期比前期少。
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| 关 键 词： | 西瓜 酯酶 过氧化物酶 同工酶 |
Change of Isoenzymes During Adventitious Bud Differentiation of Watermelon |
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| ZHANG Li-zhen,YANG Dong-ye,QIN Xin-min.Change of Isoenzymes During Adventitious Bud Differentiation of Watermelon[J].Northern Horticulture,2011(14):108-110. |
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| Authors: | ZHANG Li-zhen YANG Dong-ye QIN Xin-min |
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| Institution: | ZHANG Li-zhen1,YANG Dong-ye2,QIN Xin-min3(1.Guilin Normal College,Guilin,Guangxi 541004,2.Guilin Medical University,3.College of Life Science,Guangxi Normal University,Guangxi 541004) |
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| Abstract: | Peroxidase isoenzymes and esterase isoenzymes during adventitious bud differentiation of hua-ling water-melon were analyzed by using polyacrylamide gel electrophoresis(PAGE)and staining with benzidine reagent.The results showed that while the adventitious bud splits up,peroxidase isoenzymes changed greatly,enzyme content for 20 days reach peak value,and late differentiation appear many bands and heavy content than earlier stage generally.Esterase isoenzymes little change.The bands was more in later stage th... |
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| Keywords: | watermelon esterase peroxidase isoenzymes |
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