| 野生软枣猕猴桃离体培养体系的建立及优化 |
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| 引用本文： | 朴一龙,朴日子.野生软枣猕猴桃离体培养体系的建立及优化[J].北方园艺,2011(15):157-160. |
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| 作者姓名： | 朴一龙 朴日子 |
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| 作者单位： | 延边大学农学院,吉林延吉,133000;延边大学农学院,吉林延吉,133000 |
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| 基金项目： | 国家自然科学基金资助项目(31060254) |
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| 摘 要： | 以长白山野生软枣猕猴桃茎段作为外植体,采用6-BA配合NAA以及ZT配合NAA 2种组合研究了适于野生软枣猕猴桃离体再生的类型和植物生长调节剂组合,筛选各培养阶段高频、稳定的适宜培养基,建立了野生软枣猕猴桃离体培养快繁体系.结果表明:外植体以侧芽为佳.6-BA 1.0 mg/L+NAA 0.5 mg/L组合的MS培养基对不定芽分化最为有利,分化率可达95.83％.分化后得到的不定芽在6-BA2.0 mg/L +NAA 0.05 mg/L的MS培养基上平均每外植体分化不定芽数达5.86个.适宜的生根培养基为1/2MS+IBA 0.1mg/L,生根率高达100％.采用2步移栽法进行移栽时,第1次移栽以河沙为基质移栽成活率可达96.67％,第2次移栽成活率98％.
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| 关 键 词： | 软枣猕猴桃 离体培养 茎段 培养基 |
Establishment and Optimization of the Tissue Culture System of Wild Actinidia argula |
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| PIAO Yi-long
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PIAO Ri-zi.Establishment and Optimization of the Tissue Culture System of Wild Actinidia argula[J].Northern Horticulture,2011(15):157-160. |
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| Authors: | PIAO Yi-long PIAO Ri-zi |
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| Institution: | PIAO Yi-long,PIAO Ri-zi(College of Agricultural,Yanbian University,Yanji,Jilin 133000) |
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| Abstract: | Stem segments were used as explants,the tissue culture system of the Changbai mountain wild Actinidia argula were established.To achieve an in vitro system,the hormone combination of 6-BA and NAA was compared with combination of ZT and NAA on the stem explants and the tube rapid propagation was studied.The results showed that the best explants was lateral buds;the appropriate media for generation was MS combined with 6-BA 1.0 mg/L and NAA 0.5 mg/L,the generation rates were 95.83%;The multiple of the adventi... |
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| Keywords: | Actinidia argula tissue culture media stem segments |
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