两种提取红松种子蛋白质的工艺方法比较研究

为寻找新的植物蛋白资源，研究发现，红松种子中蛋白质含量在13％～20％，具有明显的生理活性，是一种新的具有活性的植物蛋白质资源。以脱脂红松种子粉为原料，比较PBS-NaCl缓冲液盐溶法和NaOH碱溶法提取红松种子蛋白质工艺。分别通过单因素和正交试验，确定最佳的提取工艺：研究了料液比、提取温度、提取时间、溶液浓度对蛋白质提取率的影响，并进行了L9（4^5）的正交试验，并测定了两种工艺得到的红松种子蛋白的等电点。PBS-NaCl缓冲液盐溶法得到的红松种子蛋白等电点pI=3．7，碱溶法提取蛋白的等电点pI=4．0。PBS-NaCl缓冲液盐溶法提取红松种子蛋白质的提取工艺：在温度30℃水浴环境下，以0．12mol／LPBS-NaCl缓冲液盐溶液提取，按1：25的料液比搅拌浸提150min，蛋白提取率达到62．86％。碱溶法提取红松种子蛋白质的最佳工艺参数是以0．0005mol／LNaOH溶液，按1：30料液比，在45℃条件下低速搅拌浸提60min，此时蛋白的提取率为72．07％。结论：从蛋白质提取率的角度考虑，碱溶法优于PBS-NaCl缓冲液盐溶法；从蛋白质稳定性和提取率两方面考虑，PBS-NaCl缓冲液盐溶法优于碱溶法。

Comparative Study on Two Methods of Pine Seed Protein Extraction Technology

This paper aimed at comprehensive utilization of pine, comparative study on two methods of pine seed protein extraction technology by PBS-NaCl and NaOH solution, and the effects of solid-liquid ratio, temperature,time and concentration of solution were investigated,On the basis of single factor and the orthogonal test, the optimal working parameterts were worked out. The optimal extracting of PBS-NaCl solution conditions of protein were：solid-liquid ratio was 1：25,30℃ ,0.12mol/L and extraction for 150min, the extraction rate reaches 62. 86%. The optimal extracting of NaOH solution conditions of protein were：solid-liquid ratio was 1 ： 30,45℃ ,0. 0005mol/L and extraction for 60rain,the extraction rate reaches 72.07%. Conclusion： consideration of protein extraction rate the solution of PBS-NaCl was better than NaOH solution;From the extraction of protein and the stability of protein,the solution of PBS-NaCl was best.