| 野生药用植物遏兰菜的组织培养研究 |
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| 引用本文： | 孟双,李菊,周炫,张叶军,姜长阳.野生药用植物遏兰菜的组织培养研究[J].辽宁农业科学,2010(4):19-21. |
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| 作者姓名： | 孟双 李菊 周炫 张叶军 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁,大连,116029 |
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| 基金项目： | 辽宁省高等教育教学改革研究项目，辽宁师范大学教学改革项目 |
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| 摘 要： | 以遏兰菜的子叶和下胚轴为材料,进行了芽的诱导分化与生长,生长芽的生根、试管苗移栽和移植的研究。结果证明:MS+BA 1.0 mg/L+NAA 0.1 mg/L是子叶和下胚轴芽诱导分化培养的理想培养基;MS+BA0.3 mg/L+NAA0.1 mg/L+GA30.5 mg/L是分化芽生长培养的理想培养基;1/2MS+NAA0.1 mg/L+IAA0.2 mg/L是生根培养的理想培养基;在温室中试管苗易移栽成活;移植到生长地的试管苗保持了遏兰菜的所有生物学特征。
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| 关 键 词： | 遏兰菜 愈伤组织 无性系 |
Study on Tissue Culture of Liriope spicata |
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| MENG Shuang,LI Ju,ZHOU Xuan,ZHANG Yejun,JIANG Chang-yang.Study on Tissue Culture of Liriope spicata[J].Liaoning Agricultural Sciences,2010(4):19-21. |
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| Authors: | MENG Shuang LI Ju ZHOU Xuan ZHANG Yejun JIANG Chang-yang |
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| Institution: | (College of Life Science,Liaoning Normal University,Dalian Liaoning 116029) |
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| Abstract: | The cotyledon and hypocotyledonary axis were usded as materials to study on the induction and differentiation of buds,rooting and transplanting of tube seedlings.The results suggested that the ideal medium for induction and differentiation of cotyledon and hypocotyledonary axis was MS＋ BA 1.0 mg/L＋NAA 0.1 mg/L,the ideal medium for development of buds and rooting culture was MS＋BA 0.3 mg/L＋NAA 0.1 mg/L＋GA 30.5 mg/L and 1/2MS＋NAA 0.1 mg/L＋IAA 0.2 mg/L respectively.In the glasshouse,the tube seedlings could be easily transplanted.The botanical characters could be all kept even the tube seedlings transplanted to the field. |
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| Keywords: | Thlaspi arvens Callus Clone |
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