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柏树火焰层孔菌菌丝体的液体培养
引用本文:苏 珏,贺新生,郑林用. 柏树火焰层孔菌菌丝体的液体培养[J]. 林业科学研究, 2010, 23(1): 98-101
作者姓名:苏 珏  贺新生  郑林用
作者单位:1. 西南科技大学生命科学与工程学院,四川,绵阳,621010
2. 四川农业科学院,四川,成都,610000
基金项目:四川省科技厅平台建设"农业微生物菌种资源标准化整理、整合及共享服务"项目资助和"西南科技大学研究生创新基金"资助。
摘    要:
柏树火焰层孔菌是柏树的病原菌物,是中国和亚洲的新记录属中的新记录种。本文采用液体发酵的方法对菌丝体生长的营养条件等生物学特性进行了研究。结果表明,柏树火焰层孔菌菌丝体生长最适碳源为淀粉,最适氮源为麸皮,P、K、Mg对其菌丝的生长具有促进作用,Fe、Zn对其菌丝生长具有显著抑制作用。通过生长曲线试验得到Logistic方程:y=0.22/(1+ 1.019 e-0.013x),菌丝体干质量为0.22 g·100 mL-1

关 键 词:柏树火焰层孔菌  液体培养  菌丝体产量

The Liquid Cultivation of the Mycelia of Pyrofomes demidoffii
SU Jue,HE Xin-sheng and ZHENG Lin-yong. The Liquid Cultivation of the Mycelia of Pyrofomes demidoffii[J]. Forest Research, 2010, 23(1): 98-101
Authors:SU Jue  HE Xin-sheng  ZHENG Lin-yong
Affiliation:School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan,China;School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan,China;Sichuan Academy of Agricultural Science, Chengdu 610000, Sichuan, China
Abstract:
Pyrofomes demidoffii is a new recorded species of the new recorded genus in the China and Asia, and a pathogenic fungus of juniper. Adopting the liquid fermentation to study its biological characteristics such as vegetal nutrition condtion of mycelia, the results indicated that the mycelia of P. demidoffii could be produced by liquid fermentation process. Starch and bran were the optimum carbon and nitrogen source, and P, K, Mg had the effection of acceleration to the growth of mycelia, Fe, Zn inhibited its growth. The growth curve of the logistic equation was deduced: y=0.22/(1+1.019e-0.013x), the weight of the dry mycelia was 0.22 g·100 mL-1.
Keywords:Pyrofomes demidoffii  liquid cultivation  mycelia yield
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