中国樱桃‘对樱桃’不定根离体再生植株的研究

 采用中国樱桃‘对樱桃’( Prunus pseudocerasus) 无菌生根苗的不定根作为外植体, 成功诱导了胚性愈伤组织, 实现了通过分化不定芽和诱导初生体细胞胚两种方式再生植株。根切段在MS + NAA1.0 mg/L + BA 0.05 mg/L + IBA 0.05 mg/L 培养基诱导获得胚性愈伤组织, 诱导频率达54.2 % , 该愈伤组织在MS + NAA 1.0 mg/L + BA 0.5 mg/L 培养基上不定芽分化率为100 % , 不定芽在MS + BA 0.5 mg/L + IBA 0.1mg/L 培养基上生长发育良好, 转入MS + NAA 0.02 mg/L + IBA 0.02 mg/L 生根培养基生根率达100 %; 整体不定根系统在MS 附加2 ,4-D 1.0～3.0 mg/L 和BA 0.5 mg/L 的培养基上同时诱导初生体细胞胚发生和单极不定芽发生, 每外植体上平均体细胞胚数5～25 个, 不定芽3～22 个。体细胞胚转到MS + BA 0.5 mg/L +IBA 0.1 mg/L 培养基上发育为完整植株, 植株再生率100 %。

Plant Regeneration from in Vitro Adventitious Roots of Chinese Cherry 'Duiyingtao'(Prunus pseudocerasus)

Plant regeneration was obtained on embryogenic callus ,which derived from in vitro adventitious roots of a Chinese cherry( Prunus pseudocerasus) cultivar‘Duiyingtao’,either by adventitious bud proliferation or primary somatic embryogenesis. Embryogenic callus were induced from root segments on medium MS + NAA 1.0 mg/ L +BA 0.05 mg/ L + IBA 0.05 mg/ L ,with the frequency of 54.2 %.Adventitious bus proliferation emerged on all embryogenic callus on medium MS + NAA 1.0 mg/ L +BA 0.5 mg/ L ,the buds developed on mediumMS +BA 0.5 mg/ L + IBA 0.1 mg/ L ,and rooted on medium MS + NAA 0.02 mg/ L + IBA 0.02 mg/ L by 100 %. Primary somatic embryos and adventitious bud were induced from intact root systems simultaneously on medium MS +2 ,4-D 1-3 mg/L +BA 0.5 mg/ L , with 5 - 25 somatic embryos and 3 - 22 adventitious buds per root explant . 100 % of the somatic embryos developed into intact plantlets when transferred to medium MS +BA 0.5 mg/ L + IBA 0.1 mg/ L.