| 用物种专化DNA重复序列作分子标记检测导入小麦的大赖草染色质 |
| |
| 引用本文： | 柴守诚 刘大钧. 用物种专化DNA重复序列作分子标记检测导入小麦的大赖草染色质[J]. 作物学报, 1997, 23(6): 641-645 |
| |
| 作者姓名： | 柴守诚 刘大钧 |
| |
| 作者单位： | 南京农业大学细胞遗传研究所，江苏南京，210095 |
| |
| 基金项目： | 国家“八五”攻关，“863”项目资助 |
| |
| 摘 要： | ｐＬｒ ＮＡＵ３４４，ｐＬｒ ＮＡＵ４２６和ｐＬｒＮＡＵ６４７是我们新近从大赖草中克隆到的３个特种专化ＤＮＡ重复序列。它们都大量存在于赖草属，而小麦中却很少。Ｓｕｏｔｈｅｒｎ杂交证明，ｐＬｒＮＡＵ４２６和ｐＬｒＮＡＵ６４７对导入小麦的８条不同的大草染色体（其中３条携带有小平麦赤霉病抗性基因）和两条染色体臂部都有检测效果，据此可用其作为赖草属染色质的分子标记进一步研究和利用。
|
| 关 键 词： | DNA 重复序列 分子标记 大赖草 冰麦草 小麦 |
| 收稿时间： | 1996-05-21 |
| 修稿时间： | 1996-12-27 |
Detection of Leymus racemosus Chromatins Transf ered to Common Wheat Using Species-specific DNA Repeats as Molecular Markers |
| |
| Chai Shoucheng Liu Dajun Chen Peidu Qi Lili Li Wanlong. Detection of Leymus racemosus Chromatins Transf ered to Common Wheat Using Species-specific DNA Repeats as Molecular Markers[J]. Acta Agronomica Sinica, 1997, 23(6): 641-645 |
| |
| Authors: | Chai Shoucheng Liu Dajun Chen Peidu Qi Lili Li Wanlong |
| |
| Affiliation: | Cytogenetics Institute, Nanjing Agricultural University, Nanjing, Jiangsu,210095 |
| |
| Abstract: | Three species-specific DNA repeats, pLrNAU647, pLrNAU426 and pLr-NAU344 were isolated from Leymus racemosus in our laboratory recently. They all showed strong hybridization signal with the southern blotted DNAs of Leymus species but very weak signal with those of Triticum aestivum. pLrNAU647 and pLrNAU426 were successfully used as molecular markers in identifying 8 different L. racemosus chromosomes, of which three carry wheat scab resistant genes, and two telochromosomes transfered to common wheat. |
| |
| Keywords: | Repeated DNA sequence Molecular marker Leymus racemosus Wheat |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《作物学报》浏览原始摘要信息 |
|
点击此处可从《作物学报》下载全文 |
