从中药方剂中提取的复合多糖对雏鸡免疫功能的影响

 480只7日龄健康罗曼雏鸡随机分为4组，每组120只。分别于8日龄注射中药复合多糖加环磷酰胺、中药复合多糖、环磷酰胺、生理盐水。分别在试验前、试验后第8、13、21、29、37天采血（每组15只）并涂片，测定淋巴细胞百分率及ANAE+ 淋巴细胞百分率；分别在用药前和停药后采血（每组12只）测定中性粒细胞吞噬力；分别在鸡18、25、32日龄（每组12只）测定腹腔巨噬细胞的吞噬功能；分别在NDⅡ系疫苗首免后7、14、21 d，二免后7、14、28 d采血（每组15只）分离血清，测定ND-HI抗体效价；分别在鸡4、6、9周龄（每组12只）时，测定法氏囊、胸腺、脾的重量；分别在用药前和停药后第4、8天采血（每组15只）测定红细胞免疫功能。试验结果表明：中药复合多糖可使淋巴细胞百分率和ANAE+ 淋巴细胞百分率、外周血中性粒细胞吞噬率和吞噬指数、腹腔巨噬细胞吞噬率和吞噬指数、首免和二免后ND-HI抗体效价、法氏囊和胸腺及脾重量、红细胞-C3b 花环率和红细胞-IC花环率明显升高。环磷酰胺可使上述指标明显下降，中药复合多糖加环磷酰胺组与环磷酰胺组之间上述指标差异显著，中药复合多糖加环磷酰胺组，上述指标接近或略高于生理盐水对照组。

Effects of Compound Polysaccharide Extracted from Traditional Chinese Medical Herbs on the Immunity Function in Chickens

Four hundred and eighty 7-day-old chickens(Roman) were randomly divided into four groups and with 120 chidkens for each. Eight-day-old chickens were vaccined with the Chinese herbal medicine compound polysaccharide plus cyclophosphamide, the Chinese herbal medicine compound polysaccharide, cyclophosphamide, physiologic saline. All chickens (15 in each group)were bled and smeared before injection, and on the 8th, 17th, 21st, 29th, 37th day post-vaccination, the percentage of lymphocyte and ANAE+ were determined. All chickens (12 in each group) were bled before using medicine and after stopping use medicine, the phagocytosis ability of neutrophil exythrocyte were determined. At 18-, 25- and 32-day-old of age chickens' (12 in each group) phagocytosis function of the abdominal cavity macrophage was determined. At 7th, 14th, 21st day post the frist immunization and at 7th, 14th, 28th day post the second immunization of ND Ⅱ vaccine, all chickens (15 in each group) were bled and serum was sampled, ND-HI antibody titers were determined. At 4-, 6- and 9-week-old of age chickens'(12 in each group)weight of bursa F, thymas and spleen were determined. All chickens (15 in each group) were bled before using medicine, and 4, 8 days after stopping use medicine, immunity function of red blood cell were determined. The results showed that the Chinese herbal medicine compound polysaccharide increased the percentage of lymphocyte transformations and ANAE+, the phagocytosis rate and index of neutrophil erythrocyte, the phagocytosis rate and index of the abdominal cavity macrophage, ND-HI antibody titers after the first and the second immunization, weight of bursa F, thymas and spleen, RBC-C3b rosette rate and RBC-IC rosette rate. Nevertheless, cyclophosphamide decreased all of these values. Significant differences between the group treated with the Chinese herbal medicine compound polysaccharide plus cyclophosphamide and the group treated with cyclophosphamide in these values. The values of group treated with the Chinese herbal medicine compound polysaccharide plus cyclophosphamide were higher than the control group.