| 柑橘体细胞杂种超低温保存后的植株再生 |
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| 引用本文： | 王子成,邓秀新.柑橘体细胞杂种超低温保存后的植株再生[J].园艺学报,2004,31(2):215-216. |
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| 作者姓名： | 王子成 邓秀新 |
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| 作者单位： | (华中农业大学作物遗传改良国家重点实验室，武汉430070) |
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| 基金项目： | 国家自然科学基金资助项目(30123001)，国家博士点基金项目 |
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| 摘 要： | 对9年生印度酸橘(Citrus reticaulata)和飞龙枳(Poncirus trifoliata)的体细胞杂种的茎段进行离体培养得到再生植株，用改良的玻璃化法对再生植株茎尖进行超低温保存后获得92％的再生率。这是柑橘体细胞杂种茎尖超低温保存的首例报道。
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| 关 键 词： | 柑橘 体细胞杂种 离体培养 超低温保存 |
| 文章编号： | 0513-353X(2004)02-0215-02 |
| 收稿时间： | 2003-5-27 |
| 修稿时间： | 2003-7-28 |
Plant Regeneration from the Cryopreserved Somatic Hybrid of Citrus |
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| Wang Zicheng and Deng Xiuxin.Plant Regeneration from the Cryopreserved Somatic Hybrid of Citrus[J].Acta Horticulturae Sinica,2004,31(2):215-216. |
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| Authors: | Wang Zicheng and Deng Xiuxin |
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| Institution: | (The National Key Laboratory ofCrop Genetic Improvement，Huazhong Agricultural University，Wuhan 430070，China) |
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| Abstract: | The allotetraploid citrus somatic hybrid is a new kind of germplasm created via protoplast fusion. We have succeeded in culturing the stems of 9 year-old tree of the somatic hybrid of Cleopatra Mandarin ( Citrus reticaulata) and Flying Dragon (Poncirus trifoliata) in vitro, later cryopreserved its shoot tips from in vitro and got regeneration rate by 92% of the cryopreserved shoot-tips. This is the first report on the cryopr-eservation of citrus somatic hybrid shoot-tips. |
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| Keywords: | Citrus Somatic hybrid Culture in vitro Cryopreservation |
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