| 玉米C_4型全长pepc基因导入普通小麦的研究 |
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| 引用本文： | 张庆琛,许为钢,胡 琳,李 艳,张 磊,齐学礼.玉米C_4型全长pepc基因导入普通小麦的研究[J].麦类作物学报,2010,30(2):194-197. |
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| 作者姓名： | 张庆琛 许为钢 胡 琳 李 艳 张 磊 齐学礼 |
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| 作者单位： | 1. 河南农业大学农学院,河南郑州,450002;河南省农业科学院小麦研究中心,河南郑州,450002
2. 河南省农业科学院小麦研究中心,河南郑州,450002 |
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| 基金项目： | 国家“973计划”前期专项（2009CB126009）；国家自然科学基金项目（30971785）；国家转基因生物新品种培育科技重大专项（2008ZX08002003）；河南省杰出人才计划项目（084200410005）；河南省基础与前沿技术研究计划项目（082300430090）。 |
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| 摘 要： | 为了获得具有类似C4光合途径的转pepc(磷酸烯醇式丙酮酸羧化酶)基因小麦材料,采用基因枪法将玉米C4型全长pepc基因导入小麦材料01H186-20-24,经在含4 mg.L-1L-PPT(L-phosphinothricin,草丁膦)的培养基上筛选,获得抗性再生植株,经PCR检测获得118株T0代阳性植株;进一步利用PCR对T1代植株进行筛选,T2代在隔离条件下种植于大田,Southern blot、SDS-PAGE检测表明,外源pepc基因在小麦基因组中得以整合和表达。测定了40个转基因T2代植株和受体对照的净光合速率(Pn)、PEPC活性,结果表明,在大田自然条件下,82.5%的转基因小麦的Pn增加,最大提高18.57%,达到了28.1μmol CO2.m-2.s-1,转基因植株中PEPC活性为对照的1.5~1.98倍。
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| 关 键 词： | 小麦 玉米C4型pepc基因 转基因 光合速率 PEPC活性 |
Development of Transgenic Wheat Plants with Maize C4 specific
pepc Gene by Particle Bombardment |
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| ZHANG Qing chen,XU Wei gang,HU Lin,LI Yan,ZHANG Lei,QI Xue li.Development of Transgenic Wheat Plants with Maize C4 specific
pepc Gene by Particle Bombardment[J].Journal of Triticeae Crops,2010,30(2):194-197. |
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| Authors: | ZHANG Qing chen XU Wei gang HU Lin LI Yan ZHANG Lei QI Xue li |
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| Institution: | 1. Agronomy College of Henan Agricultural University, Zhengzhou, Henan 450002, China; 2. Wheat Research Center,
Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China |
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| Abstract: | Transformation and high efficiency expression of the C4-specific pepc(phosphoenolpyruvate carboxylase) gene in wheat is an important approach to improve wheat photosynthetic character and biomass yield.In order to obtain PEPC transgenic wheat with similar C4 photosynthetic pathways,recombination binary vector pPC46 carrying maize C4-specific full-length pepc gene was transformed into immature embryos of wheat line 01H186-20-24 by particle bombardment.A total of 118 contemporary transgenic wheat plants(T0) w... |
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| Keywords: | Wheat (Triticum aestivum L ) Maize (Zea mays) C_4-specific pepc gene Transgenic wheat Photosynthetic efficiency PEPC enzyme activity |
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