Effect of excess levels of lysine and leucine in wheat‐based,amino acid‐fortified diets on the mRNA expression of two selected cationic amino acid transporters in pigs |
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Authors: | A Morales M A Barrera A B Araiza R T Zijlstra H Bernal M Cervantes |
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Institution: | 1. Instituto de Ciencias Agrícolas, UABC, Mexicali, México;2. Departamento de Agricultura y Ganadería, UNISON, Hermosillo, México;3. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada;4. Facultad de Agronomía, UANL, Monterrey, México |
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Abstract: | An experiment was conducted to evaluate the effect of excess levels of Leu and Lys on the expression of b0,+ and CAT‐1 mRNA in jejunum, liver and the muscles Longissimus dorsi (LDM) and Semitendinosus (STM). Twenty pigs with an average initial BW of 16.4 ± 1.7 kg were used in a Randomized Complete Block. Dietary treatments (T) were as follows: T1, basal diet; T2, basal plus 3.5 g l ‐Lys/kg diet; T3, basal plus 1.5 g l ‐Leu/kg diet; T4, basal plus 3.5 g l ‐Lys plus 1.5 g l ‐Leu/kg diet. Diets in T1 and T3 met 100% the requirement of Lys for pigs within the 10 to 20 kg body weight range; diets in T2 and T4 contained 35% excess of Lys. Also, diets in T1 and T2 supplied 104%, whereas diets in T3 and T4 supplied 116% the requirement of Leu. The expression of b0,+ in jejunum was reduced (p = 0.002) because of the supplementation of l ‐Leu, but l ‐Lys supplementation had no effect (p = 0.738). In contrast, the expression of b0,+ in STM (p = 0.012) and liver (p = 0.095) was reduced by the high level of Lys, but Leu had no effect (p > 0.100). CAT‐1 expression in STM increased by high Lys (p = 0.023) and Leu (p = 0.007) levels. In liver, the expression of CAT‐1 substantially increased (p = 0.001) because of Lys. In conclusion, excess levels of dietary Lys and Leu affect the expression of cationic amino acid transporters, and this effect varies depending on the studied tissue. |
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Keywords: | pigs amino acids mRNA expression amino acid transporters |
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