| 甜瓜属植物种间杂交及其杂交后代SRAP分子标记鉴定 |
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| 引用本文： | 王吉明,包文风,尚建立,马双武. 甜瓜属植物种间杂交及其杂交后代SRAP分子标记鉴定[J]. 中国瓜菜, 2010, 23(5): 30-33 |
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| 作者姓名： | 王吉明 包文风 尚建立 马双武 |
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| 作者单位： | 中国农业科学院郑州果树研究所,郑州,450009 |
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| 基金项目： | 中央级公益性科研院所基本科研业务专项 |
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| 摘 要： | 以150个薄皮甜瓜品种为母本,分别与甜瓜野生近缘种角瓜和西印度瓜进行种间杂交,结果仅以编号V2和V129的薄皮甜瓜与角瓜和西印度瓜杂交后能够坐果,但与角瓜杂交后种子败育,而与西印度瓜杂交后仅果实底部有少量可育种子。采用SRAP分子标记技术对V129×西印度瓜杂交后代进行分析表明,1对引物(E14/M2)扩增出少量父本(西印度瓜)特征带,表明西印度瓜与V129甜瓜已经在DNA分子水平上发生了交换。
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| 关 键 词： | 甜瓜 种间杂交 SRAP分子标记 |
Interspecific Hybridization between Cucumis ssp.and Identification of Their Hybrids by SRAP Markers |
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| WANG Ji-ming,BAO Wen-feng,SHANG Jian-li,MA Shuang-wu. Interspecific Hybridization between Cucumis ssp.and Identification of Their Hybrids by SRAP Markers[J]. China Cucurbits And Vegetables, 2010, 23(5): 30-33 |
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| Authors: | WANG Ji-ming BAO Wen-feng SHANG Jian-li MA Shuang-wu |
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| Abstract: | 150 melon accessions were used as female parents to cross with Cucumis metuliferus and Cucumis anguria.Only melon accessions V2 and V129 set fruits,but seeds from fruits V2(V129)×C.metuliferus were aborted.A few seeds from the bottom of fruit V2(V129)×C.anguria were fertile.Sequence-related amplified polymorphism(SRAP)molecular markers were used to analyze the progeny of interspecific hybridization between C.anguria and melon V129.One pair primer (E14/M2) amplified male parent characteristic bands in the hybrid plant,suggesting that some DNA exchange had happened between C.anguria and melon V129. |
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| Keywords: | Cucumis ssp. Interspecific hybridization SRAP Markers |
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