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| 日本血吸虫22.6 ku膜相关蛋白基因的克隆及原核表达 | |
| 引用本文： | 付媛,张雪娟,赵俊龙,何永强,刘恩勇,卢福庄,江涛,冯尚连.日本血吸虫22.6 ku膜相关蛋白基因的克隆及原核表达[J].华中农业大学学报,2007,26(3):357-360. |
| 作者姓名： | 付媛 张雪娟 赵俊龙 何永强 刘恩勇 卢福庄 江涛 冯尚连 |
| 作者单位： | 1. 浙江省农业科学院畜牧兽医研究所,杭州,310021 2. 华中农业大学农业微生物学国家重点实验室,武汉,430070 3. 浙江省进出口检验检疫局,杭州,310012 4. 湖北省兽疫防治站,武汉,430064 |
| 基金项目： | 浙江省重大科技攻关项目(2004C12027)资助 |
| 摘 要： | 为了得到重组的日本血吸虫22.6 ku膜相关蛋白,研究设计了1对引物,以日本血吸虫mRNA为模板,用RT-PCR方法克隆了576 bp的22.6 ku膜相关蛋白基因片段,测序结果表明,该片段与已公布的Sj22.6基因序列(U75510)同源性达到99%。将该基因片段插入原核表达载体pGEX-KG构建了原核表达质粒pGEX-Sj22.6,在大肠杆菌BL21 codn plus中表达获得了约48 ku的融合蛋白,经Western-blot检测,该重组蛋白具有良好的免疫原性。 |
| 关 键 词： | 日本血吸虫 22.6ku膜相关蛋白 克隆表达 |
| 文章编号： | 1000-2421（2007）03-0357-04 |
| 修稿时间： | 2006-10-24 |
Molecular Cloning and Prokaryotic Expression of the Schistosoma japonicum 22.6 ku Tegumental Membrane-associated Protein |
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| FU Yuan,ZHANG Xue-juan,ZHAO Jun-long,HE Yong-qiang,LIU En-yong,LU Fu-zhuang,JIANG Tao,FENG Shang-lian.Molecular Cloning and Prokaryotic Expression of the Schistosoma japonicum 22.6 ku Tegumental Membrane-associated Protein[J].Journal of Huazhong Agricultural University,2007,26(3):357-360. | |
| Authors: | FU Yuan ZHANG Xue-juan ZHAO Jun-long HE Yong-qiang LIU En-yong LU Fu-zhuang JIANG Tao FENG Shang-lian |
| Institution: | 1. Research Institute of Animal Science and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2. National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070,China; 3. Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310021,China; 4. Hubei Veterinary Prevention and Control Station, Wuhan 430064,China |
| Abstract: | In order to get the recombinant Schistosoma japonicum 22.6 ku tegumental protein,a pair of primers was designed.The 576 bp fragment was cloned by using reverse transcirption polymerase chain reaction(RT-PCR) from the mRNA of Schistosoma japonicum.Sequence analysis revealed that this fragment shared 99% identity with the published Sj22.6 gene sequence(U75510).The recombinant expressing plasmid pGEX-Sj22.6 was constructed and subsequently expressed in E.coli BL21.A 48 ku rSj22.6 protein was obtained.The Western-blot assay suggested that the fusion protein has good immunogenicity. |
| Keywords: | Schistosoma japonicum Sj22 6 ku protein cloning and expression |
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