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| 分子标记结合田间观察快速鉴定外源DNA导入玉米的变异后代 | |
| 引用本文： | 刘芳,刘晓鑫,高树仁,袁英,孔祥梅,刘德璞,李晓辉.分子标记结合田间观察快速鉴定外源DNA导入玉米的变异后代[J].分子植物育种,2008,6(4). |
| 作者姓名： | 刘芳 刘晓鑫 高树仁 袁英 孔祥梅 刘德璞 李晓辉 |
| 作者单位： | 1. 吉林省农业科学院生物技术研究中心,长春,130124;黑龙江八一农垦大学植物科技学院,大庆,163319 2. 吉林省农业科学院生物技术研究中心,长春,130124 3. 黑龙江八一农垦大学植物科技学院,大庆,163319 |
| 基金项目： | 吉林省科技厅青年应用基础研究专项基金 |
| 摘 要： | 本研究通过花粉管通道导入技术将甜高粱总DNA导入优良玉米自交系4112和8902中,并应用RAPD标记技术从导入后代中筛选出变异后代。从306个RAPD引物中筛选出79个引物对供受体及导入后代进行鉴定,其中10个随机引物在供、受体和导入后代间有多态性。对D1和D2代进行田间茎秆糖分调查,并对D2代室内考种,发现糖分含量和穗长、穗重、轴粗、穗粒重等穗部性状都发生了明显变异。结合分子鉴定结果,最终获得了一批变异材料。 |
| 关 键 词： | 玉米 分子标记 快速鉴定 外源DNA |
Rapid Identification of Exogenous DNA Introduction Maize Variant Offspring through Molecular Markers and Field Investigation |
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| Liu Fang,Liu Xiaoxin,Gao Shuren,Yuan Ying,Kong Xiangmei,Liu Depu,Li Xiaohui.Rapid Identification of Exogenous DNA Introduction Maize Variant Offspring through Molecular Markers and Field Investigation[J].Molecular Plant Breeding,2008,6(4). | |
| Authors: | Liu Fang Liu Xiaoxin Gao Shuren Yuan Ying Kong Xiangmei Liu Depu Li Xiaohui |
| Institution: | Liu Fang 1,2 Liu Xiaoxin 1 Gao Shuren 2 Yuan Ying 1 Kong Xiangmei 1 Liu Depu 1 Li Xiaohui 1 1 Center of Agri-Biotechnology,Jilin Academy of Agricultural Sciences,Changchun,130124,2 College of Science , Technology of Planting,Heilongjiang August First L, Reclamation University,Daqing,163319 |
| Abstract: | The total DNA of sorghum was introduced into maize inbred lines 4112 and 8902 by means of pollen tube pathway technique. Variation was screened among offspring by using RAPD. 79 out of 306 random primers were selected between donors and receptors, 10 random primers had polymorphism among donors, receptors and offspring. Besides investigation for sugar content of D1 and D2 offspring, D2 was carried through indoor studies. The results showed that it had obvious differences on characters of ear length, ear wei... |
| Keywords: | Maize Molecular marker Rapid identification Exogenous DNA |
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