冬荷抗腐烂病的初步鉴定及其生理变化

【目的】观察感染腐烂病冬荷的病情，研究不同品种冬荷抗腐烂病的生理代谢情况。【方法】以置于人工气候室盆栽冬荷“雪里红”、“傲霜”、“冬红花”3个品种和对照碗莲品种“红霞满天”为材料，人工接种腐烂病致病菌尖孢镰刀菌，在对照品种“红霞满天”发病率达25%时记录地下茎的发病情况，并取地下茎组织，测定过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性和总酚(TP)含量的变化。【结果】“雪里红”和“傲霜”为低抗品种，“冬红花”为高感品种；低抗品种“雪里红”和“傲霜”的POD、PPO、PAL活性和TP含量及其最大增幅均极显著高于高感品种“冬红花”；各冬荷品种POD活性呈双峰曲线变化，PPO活性和TP含量呈单峰曲线变化，PAL活性变化为“S”型曲线。【结论】冬荷各品种在抗病能力和抗病生理指标值的大小上均存在差异，但在抗病生理变化趋势上是相同的。

Initiatory resistance evaluation and physiological change of the winter lotus infected rot disease

【Objective】This experiment on disease degree and physiological indexes of winter lotus infected rot disease was conducted to explore the physiological and biochemical responses to the disease.【Method】With potted winter lotus cv." Xuelihong "," Aoshuang "," Donghonghua " and the control " Hongxiamantian " in the phytotron as test material,the artificial inoculation of fusarium was proceeded.After twenty-five percent inflection of the control,the subterranean stems were sampled to determine the activities of peroxidase,polyphenol oxidase,phenylalanine ammonia lyase and the content of total phenolic.【Result】The disease investigation result of winter lotus cultivars indicated that " Xuelihong " and " Aoshuang " had low resistance to the infection,and " Donghonghua " was highly sensitive;for all the enzyme activity,the content of total phenolic and their maximum extent of augment,the low resistance " Xuelihong " and " Aoshuang " were significantly higher than the highly sensitive " Donghonghua ";among all the cultivars of winter lotus,the activity of peroxidase showed a bimodal curve,the varieation of polyphenol oxidase activity and total phenolic content was of unimodal curve,and the activity of phenylalanine ammonia lyase showed a Sigmoid curve.【Conclusion】Among winter lotus cultivars,the disease resistance ability and physiological index value were different,but the variation trend was the same.