红曲菌GABA高产菌株的筛选及培养基的研究

目的]筛选出高产γ-氨基丁酸(γ-aminobutyric acid,GABA)的红曲菌菌株,并研究其最佳发酵条件。方法]通过液态发酵,从实验室保藏的23株红曲菌菌株中筛选出GABA产量较高的菌株,研究不同碳源、氮源及Ca2+、Mn2+、乙醇等其他添加成分对其产GABA的影响,并利用正交试验优化发酵培养基的组成。结果]筛选出GABA产量较高的菌株M-4,发现葡萄糖和谷氨酸单钠盐有利于GABA的产生,且当培养基组成为大米粉3%、葡萄糖4%、谷氨酸单钠盐2%、KH2PO40.15%、MgSO4.7H2O 0.10%时,M-4的GABA产量达474 mg/L,为优化前的4.6倍。结论]该研究可为开发富含GABA的红曲保健品提供参考。

Screening of High GABA-producing Monascus Strains and Optimization of Fermentation Culture

Objective] To screen high GABA-producing Monascus strains and study the optimal fermentation condition.Method] The GABA production of 23 Monascus strains kept in our laboratory was investigated by liquid fermentation and a better strain M-4 was obtained.Then the influence of different carbon and nitrogen resources,as well as other compounds such as Ca2+,Mn2+,ethanol and so on,on GABA production of M-4 was studied.The culture composition was optimized by orthogonal test.Results] Glucose and monosodium L-glutamine were preferred for M-4 to produce GABA,and the optimal medium components were as follows: rice powder 3%,glucose 4%,monosodium L-glutamine 2%,KH2PO4 0.15%,MgSO4·7H2O 0.10%.In this culture,the yield of GABA was 474 mg/L,about 4.6 times as much as that before optimizing.Conclusion] The study will provide reference for development of Monascus products with high content of GABA.