| 敖汉细毛羊体侧部与腹股沟部皮肤免疫基因的差异表达研究 |
| |
| 引用本文： | 赵金山,李和刚,刘开东,柳楠,李金泉.敖汉细毛羊体侧部与腹股沟部皮肤免疫基因的差异表达研究[J].安徽农业科学,2012(30):14731-14733,14740. |
| |
| 作者姓名： | 赵金山 李和刚 刘开东 柳楠 李金泉 |
| |
| 作者单位： | 内蒙古农业大学,内蒙古呼和浩特010018;山东省青岛市畜牧兽医研究所,山东青岛266100;山东省青岛市畜牧兽医研究所,山东青岛,266100;青岛农业大学,山东青岛,266019;内蒙古农业大学,内蒙古呼和浩特,010018 |
| |
| 基金项目： | 国家绒毛用羊产业技术体系资助项目(CARS-40) |
| |
| 摘 要： | 目的]从免疫基因中挖掘调控羊毛性状的候选主效基因。方法]利用芯片技术,检测敖汉细毛羊体侧部与腹股沟部皮肤免疫基因的差异表达。结果]差异倍数在2.0以上的免疫基因共46个,并对其中6个基因进行了实时定量PCR验证,其结果表明芯片数据和实时定量PCR验证结果相符率达66.67%,说明芯片数据是可靠的。结论]免疫赦免机制可能参与羊毛的生长调控。
|
| 关 键 词： | 敖汉细毛羊 毛囊 免疫基因 差异表达 |
Study on the Differential Expression of Immune Genes between Body Side Skin and Groin Skin of Aohan Fine Wool Sheep |
| |
| Institution: | ZHAO Jin-shan et al(Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018) |
| |
| Abstract: | Objective] The research aimed to find out candidate major genes for regulating the wool traits from immune genes.Method] Using microarray technology,the differential expression of immune genes between body side skin and groin skin of Aohan fine wool sheep was detected.Result] 46 immune genes(difference multiple)>2.0) were identified and classified.And 6 of them were validated by real-time quantitative PCR.The results showed that the consistency between microarray data and real-time quantitative PCR results was 66.67%,which indicated that the microarray data was reliable.Conclusion] The mechanism might be involved in the growth regulation of wool. |
| |
| Keywords: | Aohan fine wool sheep Hair follicle Immune gene Differential expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
