| C_(18)键合磁珠固相萃取-液质联用检测蜂王浆中磺胺二甲嘧啶残留 |
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| 引用本文： | 李金峰,李颖,蒋原,薛峰,朱海.C_(18)键合磁珠固相萃取-液质联用检测蜂王浆中磺胺二甲嘧啶残留[J].安徽农业科学,2012(27):13586-13587. |
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| 作者姓名： | 李金峰 李颖 蒋原 薛峰 朱海 |
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| 作者单位： | 1. 深圳市易瑞生物技术有限公司,广东深圳,518102
2. 江苏出入境检验检疫局动植物与食品检测中心,江苏南京,210001 |
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| 基金项目： | 科技部科技型中小企业技术创新基金项目(09C26214425280);十二五国家科技支撑计划项目(2012BAK17B10);国家国际科技合作与交流专项项目(2011DFA32930) |
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| 摘 要： | 目的]建立一种应用C18键合磁珠固相萃取-液质联用检测蜂王浆中磺胺二甲嘧啶残留的方法。方法]MCX固相萃取小柱联合C18磁珠对蜂王浆进行前处理,用HPLC-串联质谱仪对净化后样本进行检测。结果]该方法的线性范围是5～80μg/kg,相关系数0.993 1。添加水平为5、10、20μg/kg时各种化合物的平均回收率范围为93%～104%,RSD值均不大于11.3%。结论]该方法简单、快速,结合自动化设备,可实现蜂王浆样品中磺胺二甲嘧啶测定前处理的自动化。
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| 关 键 词： | 固相萃取 磁珠 磺胺二甲嘧啶 超高效液相色谱-串联质谱 |
Determination of Sulfadimidine in Royal Jelly by C18-functionalized Magnetic Silica Nanoparticles Solid Phase Extraction—High Performance Liquid Chromatography—Tandem Mass Spectrometry |
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| Institution: | LI Jin-feng et al(Shenzhen Bioeasy Technology,Inc.,Shenzhen,Guangdong 518102) |
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| Abstract: | Objective] To develop a method of C18-functionalized magnetic silica nanoparticles solid phase extraction-high performance liquid chromatography-tandem mass spectrometry for determination of sulfadimidine in royal jelly.Method] The royal jelly samples were pretreated by MCX SPE column and C18-functionalized magnetic silica nanoparticles,the detection was carried out by HPLC-MS/MS.Result] The detection method showed good linear relationship in the range of 5-80 μg/kg(r=0.993 1).The recovery ranges were between 93%-104% with the relative standard deviations(RSD) below 11.3%.Conclusion] The pretreatment method is simple,fast,time-saving,and easy to realize the automation of pretreatment with the automatic equipment. |
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| Keywords: | Solid phase extraction Magnetic silica nanoparticles Sulfadimidine High performance liquid chromatography—tandem mass spectrometry |
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