| 民族药草龙药材的质量控制研究 |
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| 引用本文： | 李兵,黄志其,韦建华,卢汝梅.民族药草龙药材的质量控制研究[J].安徽农业科学,2012(27):13338-13339. |
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| 作者姓名： | 李兵 黄志其 韦建华 卢汝梅 |
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| 作者单位： | 广西中医药大学,广西南宁,530001 |
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| 基金项目： | 2011年度广西教育厅科研项目(编号:201106LX269) |
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| 摘 要： | 目的]建立草龙药材的质量控制方法。方法]采用薄层色谱法对草龙进行定性鉴别,并采用HPLC法测定草龙药材中有效成分没食子酸的含量。色谱柱为HypersilC18柱(4.6 mm×250 mm,5μm),流动相为乙腈-浓度0.05%磷酸(5∶95,V/V),流速为1.0ml/min,检测波长为270 nm。结果]草龙药材中没食子酸的薄层色谱鉴别特征明显,专属性强;在0.40～2.40μg/ml范围内,没食子酸浓度与峰面积呈良好的线性关系(R=0.999 9),平均回收率为98.36%,RSD为2.28%(n=6)。结论]建立的草龙药材定性定量测定方法简单准确,可为草龙药材的质量控制提供科学依据。
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| 关 键 词： | 草龙 质量控制 没食子酸 |
Study on Quality Control of Ludwigia hyssopifolia |
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| Institution: | LI Bing et al(Guangxi College of Traditional Chinese Medicine,Nanning,Guangxi 530001) |
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| Abstract: | Objective] To establish a quality control method of Ludwigia hyssopifolia.Method] TLC was adopted to identify and HPLC was adopted to determine effective contents of gallic acid of Ludwigia hyssopifolia,with HypersilC18(4.6 mm×250 mm,5 μm) column as analytic column and a mobile phase consisted of acetonitrle-0.05% phosphoric acid(5∶95,V/V) at a flow rate of 1.0 ml/min and the detection wavelength at 270 nm.Result] The gallic acid of Ludwigia hyssopifolia was characterized by TLC,and was highly specific.The liner of quercetin was 0.40-2.40 μg(R=0.999 9).The average recovery was 98.36%,with RSD=2.28%(n=6).Conclusion] The method is fast and accurate,and can be used for the quality control study of Ludwigia hyssopifolia. |
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| Keywords: | Ludwigia hyssopifolia Quality control Gallic acid |
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