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| 赤羽病微量病毒中和试验诊断方法的研究 | |
| 引用本文： | 刘焕章,李树清,吴东来,李健,陈健康,王巧金,陆晓东,胡永强.赤羽病微量病毒中和试验诊断方法的研究[J].中国预防兽医学报,2001,23(1):53-55. |
| 作者姓名： | 刘焕章 李树清 吴东来 李健 陈健康 王巧金 陆晓东 胡永强 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所动物疫病诊断中心, 2. 上海出入境检验检疫局, 3. 上海浦东新区兽医站，上海 201200 4. 上海出入境检验检疫局，上海 200135 5. 上海浦东新区兽医站, |
| 摘 要： | 应用引自日本的赤羽病病毒，制备了微量病毒中和试验抗原和高免阳性血清，建立了赤羽病微量病毒中和试验诊断方法，应用此方法对广东省７２４头牛血清进行了检查，阳性率为３５．３％，对上海６６５头牛血清作了检查，阳性率为６７．７％，说明这些地区曾流行过赤羽病，同时对澳大利亚当地牛１０８头进行检查，阳性率为５５．５％，与外报道一致。对澳大利亚、加拿大，美国进口牛１７３头的检查结果，全部阴性，对澳大利亚进口羊９９２头的检查结果，发现阳性１头，已得到澳方认可。 |
| 关 键 词： | 赤羽病病毒 微量病毒中和试验 论断 牛 |
| 文章编号： | 1008-0589(2001)01-0053-03 |
| 修稿时间： | 2000年7月5日 |
Development of the Microtitre Neutralization Test for Serodiagnosis of Akabane Disease |
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| Liu Huanzhang,Li shuqing,WU Donglai,LI Jian,CHEN Jiankang,Wang Qiaoquan,Lu Xiaodong,HU Yongqiang.Development of the Microtitre Neutralization Test for Serodiagnosis of Akabane Disease[J].Chinese Journal of Preventive Veterinary Medicine,2001,23(1):53-55. | |
| Authors: | Liu Huanzhang Li shuqing WU Donglai LI Jian CHEN Jiankang Wang Qiaoquan Lu Xiaodong HU Yongqiang |
| Institution: | LIU Huanzhang 1,LI Shuqing 2,WU Donglai 1,LI Jian 2,CHEN Jiankang 3,WANG Qiaoquan 2,LU Xiaodong 3,HU Yongqiang 2 |
| Abstract: | The microtitre neutralization test has been developed for detecting antibody of Akabane Virus in serum.724 serum specimens and 665 serum specimens,which were taken from cattle distributing separately in Guangdong province and shanghai,were measured.The result shows that the positive rate was 35.5%and 67.7%,which indicates Akabane disease was once epidemic in these areas.Meanwhile,108 serum specimens of cattle from Australia were detected,the positive rate was 55.5%,being concordant with that reported abroad.173 serum specimens of cattle imported from Australia,Canada,and American were tested negatively.Only one of 992 Serum samples taken from Australia sheep was positive. |
| Keywords: | Akabane virus Microtitre neutralization test |
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